Volume 1 Supplement 1

BioSysBio 2007: Systems Biology, Bioinformatics, Synthetic Biology

Open Access

A parallel algorithm for de novo peptide sequencing

BMC Systems Biology20071(Suppl 1):P61

DOI: 10.1186/1752-0509-1-S1-P61

Published: 8 May 2007

Introduction

Protein identification is a main problem in proteomics, the large-scale analysis of proteins. Tandem mass spectrometry (MS/MS) provides an important tool to handle protein identification problem. Indeed the spectrometer is capable of ionizing a mixture of peptides, essentially several copies of the same unknown peptide, dissociating every molecule into two fragments called complementary ions, and measuring the mass/charge ratios of the peptides and of their fragments. These measures are visualized as mass peaks in a mass spectrum.

There are two fundamental approaches to interpret the spectra. The first approach is to search in a database to find the peptides that match the MS/MS spectra. This database search approach is effective for known proteins, but does not permit to detect novel proteins. This second task can be dealt with the de novo sequencing that computes the amino acid sequence of the peptides directly from their MS/MS spectra.

In the de novo sequencing problem one knows the peptide mass m P , and a subset of the masses of its ions m1,...,m n , and the task is to determine a sequence Q of masses of residues such that subsets of its prefixes and suffixes give the masses in input. The solution is, in general, not unique.

Methods

We reformulate the problem in terms of searching paths in a graph. To this goal, let M P denote the set of ion masses m i in input increased with: their complementary masses m P - m i + 2, the mass of the hydrogen, 1, and of its complementary mass m P - 17. By abuse of notation, M P = {m1,...,m n }, where m i <m j if i <j.

We build a directed acyclic graph G P = (V, E) as follows. Let a node v i associate to a member m i of M P , and an edge from v i to v j if m j - m i equals the sum of residue masses.

The de novo sequencing problem consists in determining any path from v1 to v n in the graph G P .

Although there is a unique original protein, the de novo sequencing may have in general more solutions (or none). In order to choose one sequence among the possible solutions, researchers have introduced any scoring function [13] depending on the masses of the fragments in the spectra. Our algorithm can determine either the solution of maximum score according to any given function or that of maximum length.

We use 3 algorithms:

the first algorithm consists in building the graph;

the second algorithm permits to distinguish the feasible paths that start in v1 and terminate in v n among the others;

finally, the third algorithm retrieves the solution of maximum score.

Results and conclusion

The literature offers a wide range of sequential de novo sequencing algorithm, all taking O(n logn) time, at least [4, 5]. Aiming at lowering such time barrier, we proposed a work-efficient CREW (concurrent-read exclusive write) PRAM [6] algorithm for the de novo peptide sequencing that determines the maximum length sequence in O(n) time by using log n processors. Such theoretical result showed that our algorithm is clearly scalable and reaches the theoretical, ideal efficiency.

The next step we are working on is the implementation of the proposed algorithm on a parallel machine to verify such theoretical results and scalability features.

Authors’ Affiliations

(1)
Dipartimento di Scienze Matematiche e Informatiche, Università degli studi di Siena
(2)
Dipartimento di Scienze Informatiche, Università degli studi di Pisa

References

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Copyright

© Mori et al; licensee BioMed Central Ltd. 2007

This article is published under license to BioMed Central Ltd.

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