Genomic phenotyping of the essential and non-essential yeast genome detects novel pathways for alkylation resistance
© Svensson et al; licensee BioMed Central Ltd. 2011
Received: 9 June 2011
Accepted: 6 October 2011
Published: 6 October 2011
A myriad of new chemicals has been introduced into our environment and exposure to these agents can damage cells and induce cytotoxicity through different mechanisms, including damaging DNA directly. Analysis of global transcriptional and phenotypic responses in the yeast S. cerevisiae provides means to identify pathways of damage recovery upon toxic exposure.
Here we present a phenotypic screen of S. cerevisiae in liquid culture in a microtiter format. Detailed growth measurements were analyzed to reveal effects on ~5,500 different haploid strains that have either non-essential genes deleted or essential genes modified to generate unstable transcripts. The pattern of yeast mutants that are growth-inhibited (compared to WT cells) reveals the mechanisms ordinarily used to recover after damage. In addition to identifying previously-described DNA repair and cell cycle checkpoint deficient strains, we also identified new functional groups that profoundly affect MMS sensitivity, including RNA processing and telomere maintenance.
We present here a data-driven method to reveal modes of toxicity of different agents that impair cellular growth. The results from this study complement previous genomic phenotyping studies as we have expanded the data to include essential genes and to provide detailed mutant growth analysis for each individual strain. This eukaryotic testing system could potentially be used to screen compounds for toxicity, to identify mechanisms of toxicity, and to reduce the need for animal testing.
The DNA damage response in budding yeast S. cerevisiae is well characterized, especially regarding its response to the alkylating agent methyl methanesulfonate (MMS) [1–8]. In addition to the ~150 yeast proteins directly involved in DNA repair , a plethora of proteins with other biological functions are necessary for recovery after damage [1, 2]. The mechanistic relevance of many of these proteins in cellular recovery is still not fully understood. Yeast, as a eukaryotic model system, serves as an eminent tool to develop new methods to unravel pathways for modulating the toxicity of agents, especially those agents with unknown modes of action. Several tests, such as the Ames test or the RAD54-GFP Greenscreen , exist to determine the genotoxicity of compounds. However, these tests do not always reveal the agents' modes of genotoxicity or the consequential cellular responses elicited by the interactions between the agent and cellular components other than DNA. In addition, these tests are notorious for false positives in predicting the toxicity of an agent for mammalian cells, as revealed later by animal testing. To decipher the mode of toxicity by different toxicants, powerful tools such as genomic phenotyping have been developed [1, 2, 11–16]. Such methodology is used to determine growth under various conditions for an entire panel of 4,852 yeast strains with single non-essential genes deleted. Of the estimated 6,000 genes in S. cerevisiae, 80% are non-essential for growth in rich media; the remaining are essential genes that cannot be deleted and are thus more difficult to study. The subset of essential genes is more highly conserved between species  and may therefore be of more relevance in understanding how humans react to toxicants. Essential genes can be studied in hemizygous diploid strains  and in haploid strains with either conditional expression of genes or with decreased levels of transcripts [19, 20]. We have queried the essential genes in the Decreased Abundance by mRNA Perturbation (DAmP) library of haploid strains [19, 21]; transcript levels in the DAmP library were reduced by tagging the 3' UTR of the transcripts with a sequence that elicits nonsense-mediated decay .
By using arrayed assays of growing liquid cultures in a microtiter format, sensitive detection of toxicity is achieved. Previous studies using liquid assays in microtiter plates were not high throughput enough to allow screening of the entire yeast genome , and although high throughput analysis has been achieved by others, that was only by pooling strains tagged with a specific DNA sequence 'bar-code'. That method detects differences in fast-growing strains, but slow-growing strains are depleted from the pool and are thus quantified with less precision. However, this obstacle may be overcome by deep sequencing of the 'bar-codes' instead of the more common detection by microarrays [24, 25].
Here we present a sensitive yet robust and highly automated liquid culture method that we have used as a screen to reveal modes of damage recovery in a eukaryotic system. By combining our data with protein-protein interaction maps, and using databases of functional categories, we have discovered novel biological pathways important for the recovery of cells in response to toxicants. Importantly, the screen has the potential to increase our understanding of toxicity modulating pathways for many different agents. The eukaryotic testing system we present here could be used to screen novel compounds for toxicity and thus reduce the need for animal testing.
Experimental system to query genotoxic agents
For each strain, the dose that gave 50% growth inhibition (GI50) was calculated based on a total of a median of 150 measurements (10 time points × 5 doses × 2-4 replicates). Only replicates that passed the quality criterion above of R2>0.7 were included in the calculations. The GI50 for WT haploid yeast was determined as 0.01 ± 0.002% MMS (average ± s.e.m.). The GI50 for the three control strains were 0.008 ± 0.001%, 0.006 ± 0.0005%, and 0.003 ± 0.0007% for rad14Δ, rev1Δ, and mag1Δ respectively (Figure 2B). The reproducibility between experiments was high (the average R2 for the GI50 between replicates was 0.88 ± 0.03). To make a quantitative comparison of the tested strains to previous studies, all strains were categorized as showing severe, intermediate, and slight or no sensitivity to MMS. This categorization was based on the comparison between GI50 values of the tested strains, WT and the three control strains used as standards to indicate the thresholds for slight (rad14Δ), intermediate (rev1Δ) and severe (mag1Δ) sensitivity (Figure 2C).
In total, 258 (6%) of the 4,331 deletion strains that passed the quality criterion were determined to be more sensitive to MMS than WT; among these strains 18 (7.0%) showed severe, 87 (33.7%) showed intermediate, and 153 (59.3%) showed slight MMS sensitivity (Figure 2D, Table S1, Additional file 1). A much higher fraction of the DAmP strains with hypomorphic mutations in essential genes demonstrated sensitivity to MMS compared to the deletion strains. Among the 675 DAmP strains that passed the quality criterion, 222 (33%) were MMS sensitive; among these strains 13 (5.9%) showed severe, 69 (31.1%) showed intermediate, and 140 (63.1%) showed slight MMS sensitivity (Figure 2D, Table S1, Additional file 1). The environmental stress response (ESR) genes  only modestly overlap with the genes deleted in the MMS sensitive strains; the ESR genes make up 19% of the genes deleted in sensitive strains, whereas the ESR comprises 16% of the entire genome (p = 0.002). Further, the WT used here was a modified version of BY4741 (with a plasmid conferring G418 resistance). This strain was confirmed as being slightly more sensitive than the original BY4741 (p-value < 0.01, t-test). The assay has its most sensitive range in detecting strains with GI50 between 0.002 and 0.008, where the data points to calculate the GI50 cover the entire range from control growth (100%) to no growth (0%). The confidence in calculating the GI50 of resistant strains decreases, as the growth is not as inhibited by the tested doses. However, we also identified 152 strains from both libraries (145 deletion strains, 7 DAmP strains) that showed some resistance to MMS compared to WT (Figure S1, Additional file 2). The criterion for resistance is described in the Methods section. No GO term was significantly enriched (FDR<0.05) among the genes that conferred resistance when deleted. As in previous studies, this method has been unable to reproducibly identify resistant strains [1, 2].
The data was also used to calculate the time required for the cultures to demonstrate visible growth (lag time) and the MMS dependency of the lag time (Figure S2, Additional file 2). Most strains, including the four control strains had a lag time 10-0 h. "Slow-growers" were defined as having a lag time exceeding 20 h. A large fraction (40%) of the "slow-growers" came from the relatively small DAmP library. Among the sensitive strains, a significant proportion (26.0%) were "slow-growers", which is significantly higher (p < 10-47) than in the entire collection (6.7%). This observation was further confirmed by a comparison with other "slow-growers" identified elsewhere . In this set 18.6% of the sensitive strains were identified as "slow-growers", again significantly higher (p < 10-9) than in the entire collection (8.6%). The MMS dependency of the lag time represents an alternative measure of MMS sensitivity (Figure S2C, Additional file 2).
The MMS sensitivities and the lag times of the individual strains in this liquid assay are listed in Table S1 and S2 (Additional file 1).
Enriched GO terms among the toxicity-modulating genes
response to DNA damage stimulus
DNA metabolic process
cell cycle process
chromosome organization and biogenesis
mitotic cell cycle
double-strand break repair
protein-DNA complex assembly
establishment of organelle localization
Protein-protein interaction networks
DNA repair and replication
As expected, many strains deficient in DNA repair proteins are identified as MMS sensitive. In concordance with previous results [1, 2], this set of toxicity-modulating genes include members of the RAD52 epistasis group, such as RAD50, RAD51, RAD52, RAD59, RAD54 and RAD57. Together with XRS2, these gene products are required for homologous recombination repair of DNA double-strand breaks (reviewed in ). Other DNA repair pathways that were important for cellular recovery after MMS include both the base excision repair pathway (MAG1, APN1) and the nucleotide excision repair pathway (RAD14, SSL1, TFB1, RAD26), including associated factors (RAD9, RAD24, DEF1). Rad14p is the yeast homolog of damage binding protein XPA , and Ssl1p and Tfb1p are subunits of the TFIIH complex essential for NER [32, 33]. Rad9p and Rad24p are checkpoint proteins required for NER . A branch of NER, transcription coupled repair (TCR), is effective on the transcribed strand of DNA. This pathway is represented by RAD26 and DEF1. Rad26p is the yeast homolog of CSB, a DNA dependent ATPase  and Def1p is required for the ubiquitination and subsequent proteolytic degradation of RNA pol II [36, 37]. The involvement of transcription coupled repair for DNA methylation damage is surprising in light of previous reports stating that TCR does not act on methylated DNA bases in mammalian cells . The post-replication repair error-free prone pathway was also represented in our dataset, albeit by mutants that showed only slight MMS sensitivity (POL32, MMS2, RAD6, UBC13, RAD52, RAD5, RAD18).
We also identified three essential genes that encode three subunits of the Replication Factor C (RFC), namely RFC5, RFC1 and RFC3, as being important for MMS-induced damage recovery. The RFC complex is involved in both DNA repair and DNA replication, acting as a "clamp loader" to load Pol30p (the yeast homolog of PCNA) onto DNA; RFC is also thought to contribute to the maintenance of a DNA replication checkpoint during S phase .
DNA damaging agents can induce gross chromosomal rearrangements and MMS is known to induce chromosomal aberrations in the form of telomere additions and translocations . In the last few years, various genome-wide screens have shown that more than 350 genes affect the regulation of telomere length [41–43]. The RAD52 epistasis group provides a telomerase-independent mechanism of telomere maintenance, and is heavily represented among the toxicity-modulating proteins, as mentioned above. Besides the RAD52 epistasis group, deletion of other non-essential genes involved in telomere maintenance also results in MMS sensitivity. For instance, severe MMS sensitivity results upon deletion of SGS1 encoding a DNA helicase of the RecQ family that is required for recombination-mediated telomere lengthening [44, 45]. The Sgs1p N-terminal physically interacts with Top3p  and Rmi1p , two other proteins that when lacking cause severe cellular sensitivity to MMS. Intermediate sensitivity results from deletion of EST1, encoding a protein associated with the telomere template RNA sequence (TLC1 RNA) used to add TG-repeats to form telomeric DNA that is part of the telomerase complex, and is essential for effective telomerase function [43, 48]. Deletion of YKU80 also results in intermediate MMS sensitivity; this gene encodes a subunit of the Ku heterodimer, a DNA repair complex that also binds TLC1 RNA .
DAmP mutations in three essential genes related with 'telomere maintenance' were also found to result in a MMS sensitive phenotype. These essential genes were as follows: RAP1, encoding a protein that caps chromosome ends to prevent telomere fusion [49, 50]; TEL2, encoding a protein that binds specifically to single-stranded telomeric DNA repeats and is required for telomere length regulation and telomere position effect ; DDC2, encoding a protein that interacts directly with Mec1p and Mec3p that are part of the essential component of the telomere checkpoint pathway, activated in the presence of DNA damage to induce a delay in cell cycle progression .
One of the major categories of cellular functions for essential genes is RNA processing. Approximately 10% of the entire S. cerevisiae genome is involved in one of various RNA-related processes , including mRNA splicing and export, tRNA modification, translation, rRNA processing, and RNA degradation. By screening the essential genes in the DAmP library, we found the GO term 'RNA processing' highly enriched among the toxicity-modulating proteins. A total of 61 strains sensitive to MMS had defects in proteins associated with this biological process; these are integrated within 2 subnetworks (Figure 3B). One network comprised of 20 essential proteins is primarily involved in rRNA processing and ribosome biogenesis (Figure 3B). Among this set there are two proteins that convey severe sensitivity to MMS when levels are reduced, namely Rnt1p and Prp43p. Rnt1p is an RNA endonuclease and Prp43p is an RNA helicase; both are involved in cleavage of the 3'-end of pre-rRNAs [54–56]. Prp43p is also involved in the release of lariat-introns from the spliceosome processing of pre-mRNAs . As described below, many more proteins involved in mRNA splicing were shown to affect the recovery of cells from MMS-induced damage.
Seventeen proteins in the sub-networks are involved in nuclear mRNA splicing via the spliceosome (Figure 3B). mRNA splicing is a complex reaction involving dozens of proteins, and consisting of two consecutive catalytic reactions divided into three coordinated stages . Toxicity-modulating genes were found to be involved in each of the three stages as follows: in the assembly and activation of the spliceosome (CDC40, BRR2, CLF1, LSM4, LSM8, PRP40, SMX3, PRP39); in the catalysis stage (PRP4, MSL5, PRP2, PRP24, DIB, SNU56, YHC1); and in the release, disassembly and snRNP recycling stage (PRP43 and PRP22).
Toxicity-modulating genes containing introns.
Reproducing previous data
Finally, it is very important to note that despite fewer strains being identified in the liquid assay, most (71%) of the enriched functional categories (Bonferroni adjusted p-value<0.0001) in the list of toxicity-modulating genes resulting from the liquid assay (Table S3, Additional file 1) were also found in the list resulting from the reanalysis of previous dataset (Table S4, Additional file 1) (Figure 4B). The main categories uniquely present in the liquid assay can be summarized as processing of different species of RNA, whereas the liquid assay results are lacking a significant enrichment for vesicle transport genes. It should also be noted that since the mutant libraries were screened under different growth conditions (liquid versus agar) we expected to see differences in the pathways detected.
Different modes of toxicity found through growth patterns
Notably, most of the genes (75/110, 68%) mutated in the class (ii) strains are essential. GO enrichment analysis of the different classes reveals that several functional categories are enriched (Table S8-10, Additional file 1). In particular, class (i) is overrepresented by response to DNA stimulus, DNA repair and DNA replication. The most prominent groups of enrichment in class (ii) are RNA processing and cell cycle. Class (iii) has the most widely distributed functional diversity. The cluster is enriched (FDR<0.05) for 125 GO categories, representing most enriched categories found in the entire dataset of sensitive strains.
The strains in class (iii) are registered by most assays and these results correlate well with previous datasets (78% recognized in our previous study, 51/65 non-essential gene deletion strains). The strains represented in both class (i) and (ii) are expected to be more difficult to detect in assays employing just one late time point to measure sensitivity. However, although class (i) and (ii) show a smaller overlap with previous data than does class (iii), 63% of the non-essential genes in class (i) and 66% of the non-essential genes in class (ii) were in fact detected in the previous dataset that used one late time-point to assess toxicity . Thus, methods relying on a single time point have a slightly lower resolution in detecting the growth patterns of class (i) and (ii). The dynamics of the growth curves make these clusters easy to identify using the method described here.
The complete dataset is available as a database with a web-interface available at http://genomicphenotyping.mit.edu/svensson/2011 (Figure S3, Additional file 2).
In this study, we have measured growth curves after exposure to the DNA damaging agent MMS for a collection of yeast mutant strains deficient in 5,528 essential and non-essential genes. Compared to previous studies using similar genomic phenotyping [1, 2], we have expanded the data to include essential genes, and to include detailed growth analysis of each strain; growth was measured at 10 time-points after treatment with a toxicant, in biological triplicates. By testing compounds in a eukaryotic system, an estimate of the toxicity in eukaryotic cells is given, as well as details regarding the way the cell responds to the toxicant, in this case MMS. We show here that genomic phenotyping is a valuable tool to decipher the modes of toxicity conferred by a DNA damaging agent. This was demonstrated by our identification of several novel toxicity-modulating genes, including those involved in RNA processing and telomere maintenance.
The fact that the toxicity-modulating proteins are found within protein-protein interaction networks of significantly higher connectivity than expected (p > 0.001) raises our confidence that the novel candidates are truly needed for cells to recover after MMS-induced damage. This includes the proteins involved in different kinds of RNA processing. It was recently shown that certain tRNA-modifications can influence cell survival after exposure to DNA damaging agents, in both yeast and human cells [61, 62]. Here we also identify mRNA, snoRNA and tRNA splicing as being required for survival after DNA damage, even though relatively few yeast transcripts are spliced [59, 63]. From this study, it remains inconclusive whether RNA splicing in general is important for helping the cell better handle MMS-induced damage or whether the processing of a few specialized transcripts may provide MMS resistance; such specialized targets include mRNA transcripts from the MMS2, UBC13 and RAD14 genes, three DNA repair genes all of which are spliced in yeast . However this does not explain why snoRNA and tRNA splicing is required for MMS-resistance.
In addition to genes encoding mRNA, snoRNA and tRNA processing proteins, one of the prominent groups of genes resulting in MMS sensitive strains when deleted, is involved in the rRNA metabolic process, consisting of 'rRNA catabolic process' and 'rRNA processing'. Forty-one out of the 262 (16%) genes of this GO category are toxicity-modulating. The majority of the toxicity-modulating rRNA-related genes are essential in yeast (34/41), which is presumably the reason why these pathways were not identified in previous screens.
Another cellular function highlighted in this study is telomere maintenance. In yeast, many of the telomere maintenance proteins also have functions in DNA damage responses, such as Tel1p and Mec1p, which are homologs of the human ATM and ATR kinases that are activated in response to DNA damage. Yeast telomeres are maintained differently than their metozoan counterparts. The components of the mammalian shelterin complex that protects the telomere ends have no direct homologs in budding yeast, although yeast shelterin-like proteins have been described .
The fact that a substantial proportion of the MMS sensitive strains have a slow growing phenotype under normal conditions, could reflect that this subset of the "sensitive" strains are identified as a consequence of the accumulated stress exceeding a viability threshold with the additional DNA damage. However, for the majority of the sensitive strains, this is not the case.
We have further shown that the DAmP strains are very well suited to studying essential genes in this type of damage-sensitivity screening. Given the essential role of these genes, it is not surprising that reduced levels of the transcripts lead to a reduction in growth rate for several of the DAmP strains. Compared to the diploid hemizygous strains , the DAmP strains show a higher proportion of toxicity-modulating genes (data not shown). This observation is consistent with previous results using the drug methotrexate . Compared to previous studies of genomic phenotyping, the information provided by this study is richer in data sampling, thus resulting in the possibility to further dissect the modes of toxicity and differentiate between patterns of sensitivity. New modes of sensitivity can be detected through understanding of the dynamics of the growth. Types of sensitivities that could go undetected in other systems can be scored here, as demonstrated by our self-organizing map analysis. Interestingly, the majority of the genes (68%) that were present in class (ii), were essential and primarily members of the relatively small DAmP library. This pattern of MMS sensitivity that is only apparent at higher MMS doses may be explained by the fact that lower levels of transcript expressed in the DAmP may be able to maintain sufficient protein levels to handle low levels of cellular damage but then fail at higher levels of damage.
To conclude, we present here a data-driven method to reveal modes of toxicity of different agents that impair cellular growth. This eukaryotic testing system could potentially be used to screen compounds for toxicity, to identify mechanisms of toxicity, and to reduce the need for animal testing.
S. cerevisiae strain haploid BY4741, diploid BY4743 were purchased from Research Genetics. As previously described , strain BY4741 was transformed with plasmind pYE13g (American Type Culture Collection) which confers G418 resistance. Deletion, DAmP and hemizygous library were purchased from Open Biosystems. The deletion library consists of a collection of 4,852 haploid strains where each strain has a single ORF replaced with the KanMX4 module, which confers G-418 resistance. These strains are in the BY4741 background (MATa his3Δ leu2Δ met15Δ ura3Δ ).
96-well master plates containing individual deletion strains were grown to stationary phase in 150ul YPD (10 g yeast extract, 20 g peptone, 20 g dextrose/liter), containing G-418 (Sigma) at 200ug/ml. Three wells of WT yeast and three control strains with known sensitivity were added into the plates. Settled cells were resuspended and a 1600X dilution of the cell suspension was inoculated with five doses (0, 0.004, 0.008, 0.012 and 0.016%) of MMS (Sigma) using a 96-pin Hydra (Robins Scientific). Cells were incubated for 48 h at 30°C. After 12 h, the OD600 was measured every 4 h using a Victor3 (Perkin Elmer). Comparison to cultures grown in bulk revealed small differences in growth patterns (data not shown).
Files with raw data were analyzed with in-house developed scripts in R (http://www.r-project.org). The OD measurements of empty wells were subtracted from all wells. Growth curves for the 48 hours after addition of MMS were drawn for the 5 doses for the individual yeast strains. The area under the curve (AUC) was calculated for each dose (including the mock-treated sample). For each strain, the dose-specific AUC was plotted against the dose. A line was fitted by linear regression and the goodness-of-fit (R2) was used to estimate linearity of the response. The slope revealed by the regression was used to determine the dose leading to 50% growth inhibition, GI50, by GI50= -0.5/slope (Figure 1B). R-scripts to regenerate the analysis are available in supplementary material together with instructions to access the raw data (Additional file 3). The visualization of the heat maps was done in R. Self-organizing maps were implemented through the SOM package. Functional enrichment was performed in Bingo 2.0.
Sensitivity thresholds were calculated based on the average GI50 of the three control strains (mag1Δ, rev1Δ, rad14Δ ). The resistance threshold was determined as GI50_average + (GI50_average - GI50_rad14Δ).
The data is available at a searchable database http://genomicphenotyping.mit.edu/svensson/2011 (Figure S3, Additional file 2).
Reducing the number of time-points
To assess how essential it was to measure cell density every 4 hours between hour 12 and 48 of the 48 h time course, we determined the loss of information resulting from removal of the data for several time-points (Figure S4, Additional file 2). For practical reasons it is important to note that removal of several measurements at intermediate times had only a limited effect on the reproducibility of the data. The goodness-of fit was 0.97 between the full dataset (with 10 time-points) and a reduced dataset (with six time-points). The coverage was determined by the percentage of tested strains that passed the linearity criterion as R2>0.7 using the selected time-points only. Using the more practical six point time-course, the coverage was still 84% versus 89.3% with the full non-essential dataset. On the other hand, only considering single observations (at 24 or 48 h) had drastic negative effects on the reproducibility of the data.
This research was supported by Unilever and by NIH grant CA055042 and ES002109. JPS was supported by a Swedish Research Council Fellowship. LQP was supported by a Spanish Ministry of Science and Innovation Fellowship. LDS is an American Cancer Society Research Professor. The authors would like to thank Emma Wang, Peter Kemble and Siobhan McRee for technical assistance.
- Begley TJ, Rosenbach AS, Ideker T, Samson LD: Damage recovery pathways in Saccharomyces cerevisiae revealed by genomic phenotyping and interactome mapping. Molecular Cancer Research. 2002, 1 (2): 103-112.PubMed
- Begley TJ, Rosenbach AS, Ideker T, Samson LD: Hot spots for modulating toxicity identified by genomic phenotyping and localization mapping. Molecular Cell. 2004, 16 (1): 117-125.View ArticlePubMed
- Jelinsky SA, Estep P, Church GM, Samson LD: Regulatory networks revealed by transcriptional profiling of damaged Saccharomyces cerevisiae cells: Rpn4 links base excision repair with proteasomes. Molecular and Cellular Biology. 2000, 20 (21): 8157-8167. 10.1128/MCB.20.21.8157-8167.2000.PubMed CentralView ArticlePubMed
- Jelinsky SA, Samson LD: Global response of Saccharomyces cerevisiae to an alkylating agent. Proceedings of the National Academy of Sciences of the United States of America. 1999, 96 (4): 1486-1491. 10.1073/pnas.96.4.1486.PubMed CentralView ArticlePubMed
- Chang M, Bellaoui M, Boone C, Brown GW: A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage. Proceedings of the National Academy of Sciences of the United States of America. 2002, 99 (26): 16934-16939. 10.1073/pnas.262669299.PubMed CentralView ArticlePubMed
- Hanway D, Chin JK, Xia G, Oshiro G, Winzeler EA, Romesberg FE: Previously uncharacterized genes in the UV- and MMS-induced DNA damage response in yeast. Proceedings of the National Academy of Sciences of the United States of America. 2002, 99 (16): 10605-10610. 10.1073/pnas.152264899.PubMed CentralView ArticlePubMed
- Prakash L, Prakash S: Isolation and Characterization of Mms-Sensitive Mutants of Saccharomyces-Cerevisiae. Genetics. 1977, 86 (1): 33-55.PubMed CentralPubMed
- Workman CT, Mak HC, McCuine S, Tagne JB, Agarwal M, Ozier O, Begley TJ, Samson LD, Ideker T: A systems approach to mapping DNA damage response pathways. Science. 2006, 312 (5776): 1054-1059. 10.1126/science.1122088.PubMed CentralView ArticlePubMed
- Friedberg EC, Walker GC, Siede W: DNA repair and mutagenesis. 1995, Washington, D.C.: ASM Press
- Cahill PA, Knight AW, Billinton N, Barker MG, Walsh L, Keenan PO, Williams CV, Tweats DJ, Walmsley RM: The GreenScreen((R)) genotoxicity assay: a screening validation programme. Mutagenesis. 2004, 19 (2): 105-119. 10.1093/mutage/geh015.View ArticlePubMed
- Giaever G, Chu AM, Ni L, Connelly C, Riles L, Véronneau S, Dow S, Lucau-Danila A, Anderson K, André B, Arkin AP, Astromoff A, El-Bakkoury M, Bangham R, Benito R, Brachat S, Campanaro S, Curtiss M, Davis K, Deutschbauer A, Entian KD, Flaherty P, Foury F, Garfinkel DJ, Gerstein M, Gotte D, Güldener U, Hegemann JH, Hempel S, Herman Z, et al, et al.: Functional profiling of the Saccharomyces cerevisiae genome. Nature. 2002, 418 (6896): 387-391. 10.1038/nature00935.View ArticlePubMed
- Bennett CB, Lewis LK, Karthikeyan G, Lobachev KS, Jin YH, Sterling JF, Snipe JR, Resnick MA: Genes required for ionizing radiation resistance in yeast. Nature Genetics. 2001, 29 (4): 426-434. 10.1038/ng778.View ArticlePubMed
- Hillenmeyer ME, Fung E, Wildenhain J, Pierce SE, Hoon S, Lee W, Proctor M, St Onge RP, Tyers M, Koller D, Altman RB, Davis RW, Nislow C, Giaever G: The chemical genomic portrait of yeast: Uncovering a phenotype for all genes. Science. 2008, 320 (5874): 362-365. 10.1126/science.1150021.PubMed CentralView ArticlePubMed
- Ross-Macdonald P, Coelho PS, Roemer T, Agarwal S, Kumar A, Jansen R, Cheung KH, Sheehan A, Symoniatis D, Umansky L, Heidtman M, Nelson FK, Iwasaki H, Hager K, Gerstein M, Miller P, Roeder GS, Snyder M: Large-scale analysis of the yeast genome by transposon tagging and gene disruption. Nature. 1999, 402 (6760): 413-418. 10.1038/46558.View ArticlePubMed
- Parsons AB, Lopez A, Givoni IE, Williams DE, Gray CA, Porter J, Chua G, Sopko R, Brost RL, Ho CH, Wang J, Ketela T, Brenner C, Brill JA, Fernandez GE, Lorenz TC, Payne GS, Ishihara S, Ohya Y, Andrews B, Hughes TR, Frey BJ, Graham TR, Andersen RJ, Boone C: Exploring the mode-of-action of bioactive compounds by chemical-genetic profiling in yeast. Cell. 2006, 126 (3): 611-625. 10.1016/j.cell.2006.06.040.View ArticlePubMed
- Dudley AM, Janse DM, Tanay A, Shamir R, Church GM: A global view of pleiotropy and phenotypically derived gene function in yeast. Molecular Systems Biology. 2005
- Mnaimneh S, Davierwala AP, Haynes J, Moffat J, Peng WT, Zhang W, Yang X, Pootoolal J, Chua G, Lopez A, Trochesset M, Morse D, Krogan NJ, Hiley SL, Li Z, Morris Q, Grigull J, Mitsakakis N, Roberts CJ, Greenblatt JF, Boone C, Kaiser CA, Andrews BJ, Hughes TR: Exploration of essential gene functions via titratable promoter alleles. Cell. 2004, 118 (1): 31-44. 10.1016/j.cell.2004.06.013.View ArticlePubMed
- Deutschbauer AM, Jaramillo DF, Proctor M, Kumm J, Hillenmeyer ME, Davis RW, Nislow C, Giaever G: Mechanisms of haploinsufficiency revealed by genome-wide profiling in yeast. Genetics. 2005, 169 (4): 1915-1925. 10.1534/genetics.104.036871.PubMed CentralView ArticlePubMed
- Breslow DK, Cameron DM, Collins SR, Schuldiner M, Stewart-Ornstein J, Newman HW, Braun S, Madhani HD, Krogan NJ, Weissman JS: A comprehensive strategy enabling high-resolution functional analysis of the yeast genome. Nature Methods. 2008, 5 (8): 711-718. 10.1038/nmeth.1234.PubMed CentralView ArticlePubMed
- Yan Z, Costanzo M, Heisler LE, Paw J, Kaper F, Andrews BJ, Boone C, Giaever G, Nislow C: Yeast Barcoders: a chemogenomic application of a universal donor-strain collection carrying bar-code identifiers. Nature Methods. 2008, 5 (8): 719-725. 10.1038/nmeth.1231.View ArticlePubMed
- Schuldiner M, Collins SR, Thompson NJ, Denic V, Bhamidipati A, Punna T, Ihmels J, Andrews B, Boone C, Greenblatt JF, Weissman JS, Krogan NJ: Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile. Cell. 2005, 123 (3): 507-519. 10.1016/j.cell.2005.08.031.View ArticlePubMed
- Muhlrad D, Parker R: Aberrant mRNAs with extended 3 ' UTRs are substrates for rapid degradation by mRNA surveillance. Rna-a Publication of the Rna Society. 1999, 5 (10): 1299-1307.View Article
- Toussaint M, Conconi A: High-throughput and sensitive assay to measure yeast cell growth: a bench protocol for testing genotoxic agents. Nature Protocols. 2006, 1 (4): 1922-1928. 10.1038/nprot.2006.304.View ArticlePubMed
- Smith AM, Ammar R, Nislow C, Giaever G: A survey of yeast genomic assays for drug and target discovery. Pharmacology & Therapeutics. 2010, 127 (2): 156-164.View Article
- Smith AM, Heisler LE, Mellor J, Kaper F, Thompson MJ, Chee M, Roth FP, Giaever G, Nislow C: Quantitative phenotyping via deep barcode sequencing. Genome Research. 2009, 19 (10): 1836-1842. 10.1101/gr.093955.109.PubMed CentralView ArticlePubMed
- Kellis M, Patterson N, Endrizzi M, Birren B, Lander ES: Sequencing and comparison of yeast species to identify genes and regulatory elements. Nature. 2003, 423 (6937): 241-254. 10.1038/nature01644.View ArticlePubMed
- Gasch AP, Spellman PT, Kao CM, Carmel-Harel O, Eisen MB, Storz G, Botstein D, Brown PO: Genomic expression programs in the response of yeast cells to environmental changes. Molecular Biology of the Cell. 2000, 11 (12): 4241-4257.PubMed CentralView ArticlePubMed
- Boone Lab - SGA Technology. [http://www.utoronto.ca/boonelab/sga_technology/index.shtml]
- Lee I, Li Z, Marcotte EM: An improved, bias-reduced probabilistic functional gene network of baker's yeast, Saccharomyces cerevisiae. Plos One. 2007, 2 (10): e988-10.1371/journal.pone.0000988.PubMed CentralView ArticlePubMed
- Symington LS: Role of RAD52 Epistasis Group Genes in Homologous Recombination and Double-Strand Break Repair. Microbiol Mol Biol Rev. 2002, 66 (4): 630-670. 10.1128/MMBR.66.4.630-670.2002.PubMed CentralView ArticlePubMed
- Bankmann M, Prakash L, Prakash S: Yeast Rad14 and Human Xeroderma-Pigmentosum Group-a DNA-Repair Genes Encode Homologous Proteins. Nature. 1992, 355 (6360): 555-558. 10.1038/355555a0.View ArticlePubMed
- Feaver WJ, Svejstrup JQ, Bardwell L, Bardwell AJ, Buratowski S, Gulyas KD, Donahue TF, Friedberg EC, Kornberg RD: Dual Roles of a Multiprotein Complex from Saccharomyces-Cerevisiae in Transcription and DNA-Repair. Cell. 1993, 75 (7): 1379-1387. 10.1016/0092-8674(93)90624-Y.View ArticlePubMed
- Habraken Y, Sung P, Prakash S, Prakash L: Transcription factor TFIIH and DNA endonuclease Rad2 constitute yeast nucleotide excision repair factor 3: Implications for nucleotide excision repair and Cockayne syndrome. Proceedings of the National Academy of Sciences of the United States of America. 1996, 93 (20): 10718-10722. 10.1073/pnas.93.20.10718.PubMed CentralView ArticlePubMed
- Yu SR, Teng YM, Lowndes NF, Waters R: RAD9, RAD24, RAD16 and RAD26 are required for the inducible nucleotide excision repair of UV-induced cyclobutane pyrimidine dimers from the transcribed and non-transcribed regions of the Saccharomyces cerevisiae MFA2 gene. Mutation Research-DNA Repair. 2001, 485 (3): 229-236. 10.1016/S0921-8777(01)00061-1.View ArticlePubMed
- Vangool AJ, Verhage R, Swagemakers SMA, Vandeputte P, Brouwer J, Troelstra C, Bootsma D, Hoeijmakers JHJ: Rad26, the Functional Saccharomyces-Cerevisiae Homolog of the Cockayne-Syndrome-B Gene Ercc6. Embo Journal. 1994, 13 (22): 5361-5369.
- Gaillard H, Wellinger RE, Aguilera A: A new connection of mRNP biogenesis and export with transcription-coupled repair. Nucleic Acids Research. 2007, 35 (12): 3893-3906. 10.1093/nar/gkm373.PubMed CentralView ArticlePubMed
- Reid J, Svejstrup JQ: DNA damage-induced Def1-RNA polymerase II interaction and Def1 requirement for polymerase ubiquitylation in vitro. Journal of Biological Chemistry. 2004, 279 (29): 29875-29878. 10.1074/jbc.C400185200.View ArticlePubMed
- Plosky B, Samson L, Engelward BP, Gold B, Schlaen B, Millas T, Magnotti M, Schor J, Scicchitano DA: Base excision repair and nucleotide excision repair contribute to the removal of N-methylpurines from active genes. DNA Repair. 2002, 1 (8): 683-696. 10.1016/S1568-7864(02)00075-7.View ArticlePubMed
- Sugimoto K, Ando S, Shimomura T, Matsumoto K: Rfc5, a replication factor C component, is required for regulation of Rad53 protein kinase in the yeast checkpoint pathway. Molecular and Cellular Biology. 1997, 17 (10): 5905-5914.PubMed CentralView ArticlePubMed
- Stellwagen AE, Haimberger ZW, Veatch JR, Gottschling DE: Ku interacts with telomerase RNA to promote telomere addition at native and broken chromosome ends. Genes & Development. 2003, 17 (19): 2384-2395. 10.1101/gad.1125903.View Article
- Askree SH, Yehuda T, Smolikov S, Gurevich R, Hawk J, Coker C, Krauskopf A, Kupiec M, McEachern MJ: A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proceedings of the National Academy of Sciences of the United States of America. 2004, 101 (23): 8658-8663. 10.1073/pnas.0401263101.PubMed CentralView ArticlePubMed
- Gatbonton T, Imbesi M, Nelson M, Akey JM, Ruderfer DM, Kruglyak L, Simon JA, Bedalov A: Telomere Length as a Quantitative Trait: Genome-Wide Survey and Genetic Mapping of Telomere Length-Control Genes in Yeast. PLoS Genet. 2006, 2 (3): e35-10.1371/journal.pgen.0020035.PubMed CentralView ArticlePubMed
- Ungar L, Yosef N, Sela Y, Sharan R, Ruppin E, Kupiec M: A genome-wide screen for essential yeast genes that affect telomere length maintenance. Nucl Acids Res. 2009, 37 (12): 3840-3849. 10.1093/nar/gkp259.PubMed CentralView ArticlePubMed
- Lee JY, Kozak M, Martin JD, Pennock E, Johnson FB: Evidence that a RecQ helicase slows senescence by resolving recombining telomeres. Plos Biology. 2007, 5 (6): 1334-1344.View Article
- Lillard-Wetherell K, Combs KA, Groden J: BLM helicase complements disrupted type II telomere lengthening in telomerase-negative sgs1 yeast. Cancer Research. 2005, 65 (13): 5520-5522. 10.1158/0008-5472.CAN-05-0632.View ArticlePubMed
- Gangloff S, Mcdonald JP, Bendixen C, Arthur L, Rothstein R: The Yeast Type-I Topoisomerase Top3 Interacts with Sgs1, a DNA Helicase Homolog - a Potential Eukaryotic Reverse Gyrase. Molecular and Cellular Biology. 1994, 14 (12): 8391-8398.PubMed CentralView ArticlePubMed
- Chang M, Bellaoui M, Zhang CY, Desai R, Morozov P, Delgado-Cruzata L, Rothstein R, Freyer GA, Boone C, Brown GW: RMI1/NCE4, a suppressor of genome instability, encodes a member of the RecQ helicase/Topo III complex. Embo Journal. 2005, 24 (11): 2024-2033. 10.1038/sj.emboj.7600684.PubMed CentralView ArticlePubMed
- Blackburn EH: Telomere states and cell fates. Nature. 2000, 408 (6808): 53-56. 10.1038/35040500.View ArticlePubMed
- Negrini S, Ribaud V, Bianchi A, Shore D: DNA breaks are masked by multiple Rap1 binding in yeast: implications for telomere capping and telomerase regulation. Genes & Development. 2007, 21 (3): 292-302. 10.1101/gad.400907.View Article
- Pardo B, Marcand P: Rap1 prevents telomere fusions by nonhomologous end joining. Embo Journal. 2005, 24 (17): 3117-3127. 10.1038/sj.emboj.7600778.PubMed CentralView ArticlePubMed
- Runge KW, Zakian VA: TEL2, an essential gene required for telomere length regulation and telomere position effect in Saccharomyces cerevisiae. Mol Cell Biol. 1996, 16 (6): 3094-3105.PubMed CentralView ArticlePubMed
- Enomoto S, Glowczewski L, Berman J: MEC3, MEC1, and DDC2 Are Essential Components of a Telomere Checkpoint Pathway Required for Cell Cycle Arrest during Senescence in Saccharomyces cerevisiae. Mol Biol Cell. 2002, 13 (8): 2626-2638. 10.1091/mbc.02-02-0012.PubMed CentralView ArticlePubMed
- Wilmes GM, Bergkessel M, Bandyopadhyay S, Shales M, Braberg H, Cagney G, Collins SR, Whitworth GB, Kress TL, Weissman JS, Ideker T, Guthrie C, Krogan NJ: A Genetic Interaction Map of RNA-Processing Factors Reveals Links between Sem1/Dss1-Containing Complexes and mRNA Export and Splicing. Molecular Cell. 2008, 32 (5): 735-746. 10.1016/j.molcel.2008.11.012.PubMed CentralView ArticlePubMed
- Braglia P, Kawauchi J, Proudfoot NJ: Co-transcriptional RNA cleavage provides a failsafe termination mechanism for yeast RNA polymerase I. Nucl Acids Res. 2011, 39 (4): 1439-1448. 10.1093/nar/gkq894.PubMed CentralView ArticlePubMed
- Elela SA, Ares M: Depletion of yeast RNase III blocks correct U2 3' end formation and results in polyadenylated but functional U2 snRNA. Embo Journal. 1998, 17 (13): 3738-3746. 10.1093/emboj/17.13.3738.PubMed CentralView ArticlePubMed
- Pertschy B, Schneider C, Gnadig M, Schafer T, Tollervey D, Hurt E: RNA Helicase Prp43 and Its Co-factor Pfa1 Promote 20 to 18 S rRNA Processing Catalyzed by the Endonuclease Nob1. Journal of Biological Chemistry. 2009, 284 (50): 35079-35091. 10.1074/jbc.M109.040774.PubMed CentralView ArticlePubMed
- Mayas RM, Maita H, Semlow DR, Staley JP: Spliceosome discards intermediates via the DEAH box ATPase Prp43p. Proceedings of the National Academy of Sciences of the United States of America. 2010, 107 (22): 10020-10025. 10.1073/pnas.0906022107.PubMed CentralView ArticlePubMed
- Smith DJ, Query CC, Konarska MM: Nought May Endure but Mutability : Spliceosome Dynamics and the Regulation of Splicing. 2008, 30 (6): 657-666.
- Juneau K, Palm C, Miranda M, Davis RW: High-density yeast-tiling array reveals previously undiscovered introns and extensive regulation of rneiotic splicing. Proceedings of the National Academy of Sciences of the United States of America. 2007, 104 (5): 1522-1527. 10.1073/pnas.0610354104.PubMed CentralView ArticlePubMed
- Huh WK, Falvo JV, Gerke LC, Carroll AS, Howson RW, Weissman JS, O'Shea EK: Global analysis of protein localization in budding yeast. Nature. 2003, 425 (6959): 686-691. 10.1038/nature02026.View ArticlePubMed
- Begley U, Dyavaiah M, Patil A, Rooney JP, DiRenzo D, Young CM, Conklin DS, Zitomer RS, Begley TJ: Trm9-catalyzed tRNA modifications link translation to the DNA damage response. Molecular Cell. 2007, 28 (5): 860-870. 10.1016/j.molcel.2007.09.021.PubMed CentralView ArticlePubMed
- Fu D, Brophy JAN, Chan CTY, Atmore KA, Begley U, Paules RS, Dedon PC, Begley TJ, Samson LD: Human AlkB Homolog ABH8 Is a tRNA Methyltransferase Required for Wobble Uridine Modification and DNA Damage Survival. Molecular and Cellular Biology. 2010, 30 (10): 2449-2459. 10.1128/MCB.01604-09.PubMed CentralView ArticlePubMed
- Miura F, Kawaguchi N, Sese J, Toyoda A, Hattori M, Morishita S, Ito T: A large-scale full-length cDNA analysis to explore the budding yeast transcriptome. Proceedings of the National Academy of Sciences of the United States of America. 2006, 103 (47): 17846-17851. 10.1073/pnas.0605645103.PubMed CentralView ArticlePubMed
- Bonetti D, Clerici M, Anbalagan S, Martina M, Lucchini G, Longhese MP: Shelterin-Like Proteins and Yku Inhibit Nucleolytic Processing of Saccharomyces cerevisiae Telomeres. Plos Genetics. 2010, 6 (5):793-798View Article
- Giaever G, Flaherty P, Kumm J, Proctor M, Nislow C, Jaramillo DF, Chu AM, Jordan MI, Arkin AP, Davis RW: Chemogenomic profiling: Identifying the functional interactions of small molecules in yeast. Proceedings of the National Academy of Sciences of the United States of America. 2004, 101 (3): 793-798. 10.1073/pnas.0307490100.PubMed CentralView ArticlePubMed
- Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, Amin N, Schwikowski B, Ideker T: Cytoscape: A software environment for integrated models of biomolecular interaction networks. Genome Research. 2003, 13 (11): 2498-2504. 10.1101/gr.1239303.PubMed CentralView ArticlePubMed
- Maere S, Heymans K, Kuiper M: BiNGO: a Cytoscape plugin to assess overrepresentation of Gene Ontology categories in Biological Networks. Bioinformatics. 2005, 21 (16): 3448-3449. 10.1093/bioinformatics/bti551.View ArticlePubMed
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.