Figure 3

Comparison of the phenotypes in each of the alternative networks upon depletion of a given protein to the corresponding phenotype observed experimentally. Within each panel, we present the overall shapes of the curves that were obtained in our in silico simulations upon depletion of the target protein and points are presented to represent the curves of published experiments. Only in two cases were there more than two measurements made along the depletion curve. The curves are connected to the alternative networks according to the following labels. R: network where deleted protein acts only on repair. F: network where deleted protein acts only on folding. S: network where deleted protein acts only on synthesis. St: network where deleted protein acts only on stability of clusters. A: network where Grx5 acts on regulating glutathionylation state of Arh1. Any combination of labels indicates that the deleted protein in the network acts on more that one process. For example RS means that said protein acts on repair and synthesis of clusters. Panels A, C, D, E, G and I compare the accumulation of Fe in the network to that in the experiments. The Y-axis represents the level of free Fe. Panels B, D, F, H and J compare the evolution of ISC dependent protein activity in the alternative networks to that observed experimentally upon depletion of the protein. The Y-axis represents normalized ISC dependent protein activity. Panels A and B – Experimental data from [74] and [73]. Panels C and D – Experimental data from [88] and [87]. Panels E and F – Experimental data from [36]. Panels G and H – Experimental data from [125] and [126]. Panels I and J – Experimental data from [77, 127, 128].