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Table 1 Proteins involved in ISC synthesis in Saccharomyces cerevisiae.

From: In silico pathway reconstruction: Iron-sulfur cluster biogenesis in Saccharomyces cerevisiae

Proteins Protein Function Type of evidence for Protein Function Suggested Function In ISC Assembly Type of evidence for suggested systemic role
Arh1 Ferredoxin Reductase Homology to known ferredoxin/adrenodoxin reductases Reduces Yah1/Provides electrons for ISC assembly/transfer/repair Homology, Physiological, Genetic
Yah1 Ferredoxin Homology to known ferredoxin/adrenodoxin Provides electrons for ISC assembly/transfer/repair Physiological, Genetic
Yfh1 Frataxin Homology, Biochemical; Structural Stores/Provides Fe directly to ISC assembly and Heme synthesis Physiological, Structural, Genetic
Grx5 Glutaredoxin Homology, Biochemical Regulates glutathionylation state of protein cysteinyl residues Physiological, Biochemical
Isa1 Scaffold   As dimer scaffolds initial ISC assembly between two monomers and then transfers it to apo-proteins Biochemical, Spectroscopic, Direct transfer observation
Isa2 Scaffold   As dimer scaffolds initial ISC assembly between two monomers and then transfers it to apo-proteins Biochemical, Spectroscopic, Direct transfer observation
Isu1 Scaffold   As dimer scaffolds initial ISC assembly between two monomers and then transfers it to apo-proteins Biochemical, Spectroscopic, Direct transfer observation
Isu2 Scaffold   As dimer scaffolds initial ISC assembly between two monomers and then transfers it to apo-proteins Biochemical, Spectroscopic, Direct transfer observation
Nfu1 Scaffold   As dimer scaffolds initial ISC assembly between two monomers and then transfers it to apo-proteins Biochemical, Spectroscopic
Ssq1 Hsp70 Chaperone Homology, Biochemical, Genetic, Structural Assists in proper folding of ISC biosynthetic proteins, namely Yfh1 and Isa-Isu proteins/Assists in maintaining ISC assembled in scaffold dimer for proper transfer Biochemical, Structural
Jac1 Hsp40 Co- chaperone Homology, Biochemical, Genetic, Structural Assists Ssq1 in interacting with Isu/Isa proteins Biochemical, Genetic, Structural
Mge1 Co- chaperone/Nucleotide exchange factor Homology, Biochemical, Genetic, Structural Assists Ssq1 Biochemical, Genetic, Structural
Nfs1 Cysteine Desulfurase Homology, Biochemical Provides sulphur for ISC assembly in scaffold dimers or in situ ISC assembly/repair Physiological, Biochemical, Genetics
Atm1 ABC transporter Homology Involved in ISC export for cytoplasm and nuclear proteins Physiological, Genetic
Isd11 unknown Biochemical, Genetic Fundamental for Nfs1 action Physiological, Genetic