Dynamics of in silico leukocyte rolling, activation, and adhesion

BMC Systems Biology

Table 1 Targetable phenotypic attributes of leukocytes in vitro: an abridged list

Phenotypic Attribute	Figure/Table	Reference
Set A: initially targeted attributes
Jerky stop and go movement	Figures 5, 6	[16]
Highly fluctuating rolling velocities	Figure 7A	[15]
Larger rolling velocities observed at higher shear rates	Figures 6A, 8	[50]
Smaller rolling velocities at higher ligand substrate densities	Not Shown	[50]
Set B: additional attributes added to targeted set
ROLLING^a velocities on PSELECTIN^a match reported values	Table 6	[50]
Small number of bonds within the contact zone, e.g., within 2–20	Table 6	[19], [20]
Distance-time and velocity-time data for ROLLING^a on ^aPSELECTIN/VCAM1 are indistinguishable from reported data	Figures 6, 7, 8	[15], [16]
Chemokines induce adhesion within seconds	Figure 10	[3]
Leukocyte spreading during firm adhesion	Figure 10	[51]
Set C: future, targetable attributes
Ligand-Receptor mobility, valency (clustering), and trafficking	N/A	[5], [6], [52]
Change in contact surface area with increasing shear force	N/A	[25], [53]
Shear threshold effect for selectins (minimum shear required for rolling)	N/A	[50]
Effect of inhibitors to signaling molecules on cell arrest (pertussis toxin (PTx)-sensitive G proteins, phosphoinositide 3-kinase, p38 mitogen-activated protein kinase)	N/A	[3]
Intracellular calcium mobilization	N/A	[3]

During early development of the ISWBCs attention focused primarily on Set A. Once satisfactory simulations began to be achieved, the targeted attributes were expanded to those in Set B. Final validations focused on the combined set.
^a We use SMALL CAPS when referring to the in silico components (see Table 3), features, measurements, and events.

ISSN: 1752-0509