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Table 1 Targetable phenotypic attributes of leukocytes in vitro: an abridged list

From: Dynamics of in silico leukocyte rolling, activation, and adhesion

Phenotypic Attribute Figure/Table Reference
Set A: initially targeted attributes   
Jerky stop and go movement Figures 5, 6 [16]
Highly fluctuating rolling velocities Figure 7A [15]
Larger rolling velocities observed at higher shear rates Figures 6A, 8 [50]
Smaller rolling velocities at higher ligand substrate densities Not Shown [50]
Set B: additional attributes added to targeted set   
ROLLINGa velocities on PSELECTINa match reported values Table 6 [50]
Small number of bonds within the contact zone, e.g., within 2–20 Table 6 [19], [20]
Distance-time and velocity-time data for ROLLINGa on aPSELECTIN/VCAM1 are indistinguishable from reported data Figures 6, 7, 8 [15], [16]
Chemokines induce adhesion within seconds Figure 10 [3]
Leukocyte spreading during firm adhesion Figure 10 [51]
Set C: future, targetable attributes   
Ligand-Receptor mobility, valency (clustering), and trafficking N/A [5], [6], [52]
Change in contact surface area with increasing shear force N/A [25], [53]
Shear threshold effect for selectins (minimum shear required for rolling) N/A [50]
Effect of inhibitors to signaling molecules on cell arrest (pertussis toxin (PTx)-sensitive G proteins, phosphoinositide 3-kinase, p38 mitogen-activated protein kinase) N/A [3]
Intracellular calcium mobilization N/A [3]
  1. During early development of the ISWBCs attention focused primarily on Set A. Once satisfactory simulations began to be achieved, the targeted attributes were expanded to those in Set B. Final validations focused on the combined set.
  2. a We use SMALL CAPS when referring to the in silico components (see Table 3), features, measurements, and events.