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Table 5 Experimental values for the three in vitro flow chamber environments and the corresponding parameter values used for each of the three simulated experimental conditions

From: Dynamics of in silico leukocyte rolling, activation, and adhesion

  In Vitro Values ISWBC Values
Experiment Shear (dyn/cm2) Substrate Molecule Site density/Plating concentration Rear Force LIGAND LIGAND Density
I. Neutrophil Rolling on P -Selectin
A. Pause Time 0.5, 1.0, 2.0 P-selectin 9 sites/μm2 0.1–0.5 PSELECTIN 15 ± 5
B. Distance-Time 2 P-selectin 25 sites/μm2 0.5 PSELECTIN 25 ± 5
C. Velocity-Time 0.5 P-selectin 9 sites/μm2 0.1 PSELECTIN 15 ± 5
II. T-Lymphocyte Rolling on VCAM-1
Distance-Time 0.73 – 7.3 VCAM-1 15 μg/mLa 0.15–1.6 VCAM1 45 ± 5
III. Monocyte Rolling on P-selectin/VCAM- 1/GRO-α
Rolling and Adhesion 1 VCAM-1 100 ng/mLa 1 VCAM1 13 ± 5
   P-Selectin 10 μg/mLa   PSELECTIN 25 ± 5
   GRO-α 5 μg/mLa   GROA 3 ± 2
  1. The FLOW CHAMBER SURFACE dimensions were fixed at 100 × 60 SURFACE UNITS. Each SURFACE grid space maps to approximately 1 μm2 of effective flow chamber surface area. a Values listed in units of [μg/mL] are the concentrations of the solutions of receptors used to coat the parallel plate flow chamber surfaces and are not meant to represent the concentrations of receptors found on the parallel plate surface.