Dynamics of in silico leukocyte rolling, activation, and adhesion

BMC Systems Biology

Table 5 Experimental values for the three in vitro flow chamber environments and the corresponding parameter values used for each of the three simulated experimental conditions

	In Vitro Values			ISWBC Values
Experiment	Shear (dyn/cm²)	Substrate Molecule	Site density/Plating concentration	Rear Force	LIGAND	LIGAND Density
I. Neutrophil Rolling on P -Selectin
A. Pause Time	0.5, 1.0, 2.0	P-selectin	9 sites/μm²	0.1–0.5	PSELECTIN	15 ± 5
B. Distance-Time	2	P-selectin	25 sites/μm²	0.5	PSELECTIN	25 ± 5
C. Velocity-Time	0.5	P-selectin	9 sites/μm²	0.1	PSELECTIN	15 ± 5
II. T-Lymphocyte Rolling on VCAM-1
Distance-Time	0.73 – 7.3	VCAM-1	15 μg/mL^a	0.15–1.6	VCAM1	45 ± 5
III. Monocyte Rolling on P-selectin/VCAM- 1/GRO-α
Rolling and Adhesion	1	VCAM-1	100 ng/mL^a	1	VCAM1	13 ± 5
		P-Selectin	10 μg/mL^a		PSELECTIN	25 ± 5
		GRO-α	5 μg/mL^a		GROA	3 ± 2

The FLOW CHAMBER SURFACE dimensions were fixed at 100 × 60 SURFACE UNITS. Each SURFACE grid space maps to approximately 1 μm² of effective flow chamber surface area. ^a Values listed in units of [μg/mL] are the concentrations of the solutions of receptors used to coat the parallel plate flow chamber surfaces and are not meant to represent the concentrations of receptors found on the parallel plate surface.

ISSN: 1752-0509