Figure 11From: A hierarchical approach employing metabolic and gene expression profiles to identify the pathways that confer cytotoxicity in HepG2 cellsComplex I inhibitor decreases LDH release. HepG2 cells were pretreated with the inhibitor of mitochondrial complex I- rotenone (0.5 μM) for 30 minutes, and then exposed to 0.7 mM palmitate for 24 h. After the treatment, the media were collected and LDH released was measured. The cells were lyzed in 1% triton for 24 h to obtain the unreleased LDH. The LDH released was normalized to the total LDH (released + unreleased). Data presented as average +/- s.d. of n = 9 from three independent experiments. *, significantly lower than palmitate.Back to article page