Figure 15From: A hierarchical approach employing metabolic and gene expression profiles to identify the pathways that confer cytotoxicity in HepG2 cellsRNA interference of EMP3. Cells were transfected with siRNA targeting EMP3, control cells were mock-transfected. The transfected cells were cultured in antibiotic-free medium for 24 h, followed by culture in medium containing 4% BSA for 24 h. Total RNA was extracted and the transcript levels of EMP3 were measured by RT-PCR. Data presented as mean +/- s.d. of n = 9 from 3 independent experiments.Back to article page