LDH release measurement of cytotoxicity of palmitate. Confluent HepG2 cells were treated with saturated (palmitate), monounsaturated (oleate) and polyunsaturated (linoleate) free fatty acid at 0.7 mM in presence or absence of TNF-α (0, 20 and 100 ng/ml) for 24 h. Cytotoxicity was measured as % LDH released. Data presented as average +/- s.d. of n = 9 from three independent experiments. *, significantly greater than control; #, significant TNF-α interaction effect (significantly greater than palmitate culture in absence of TNF-α. For both * and #, p < 0.01.