Figure 5

Comparison between measured and predicted flux rates of E. coli. x-axis: observed flux rates measured by Emmerling et al. [36]. y-axis: predicted flux rates obtained with three different variants of FBA. Fluxes were transformed into dimensionless units by putting the highest observed and calculated value equal to unity. All flux distributions were obtained by using the maximization of biomass as flux objective. Blue triangles: All reactions are fully reversible (except exchange fluxes and 37 internal fluxes with unknown value of free energy changes); solution of the complete optimization problem (eq. 10), i.e. condition of thermodynamic realizability included. Red squares: All reactions are fully reversible (except the exchange fluxes and 37 internal fluxes with unknown value of free energy changes); solution to biomass maximization at fixed glucose intake; the condition of thermodynamic realizability was omitted. Green diamonds: Irreversibility of 532 reactions prescribed as originally proposed by Reed et al. [25]; solution to biomass maximization at fixed glucose intake. Coefficients marked with r = denote the Pearson's product-moment correlation coefficient.