Integration of enzyme activities into metabolic flux distributions by elementary mode analysis

BMC Systems Biology

Table 2 Experimental data of flux and enzyme activities in wild type and the pykF(-) knockout mutant

	Flux in the wild type	Flux in the mutant	Relative enzyme activities in the pykF(-) mutant
1	100	100	0.57
2	83	65	0.57
3	163	151	1
4	30	1	0.14
5	107	103	1
6	20	1	0.37
7 (gltA)	87	81	0.45
8	87	81	1
9	78	73	1
10	75	52	1.47
11	17	44	2.3
12	3	21	3.5
13	34	79	1.7
14	34	79	1.44
15	11	25	1
16	8	22	1
17	11	25	1
18	65	20	0.59
19	100	100	1
20	1	1	1
21	1	2	1
22	11	1	1
23	16	6	1
24	26	19	1
25	1	21	1
26	19	1	1
27	9	8	1
28	5	15	1
29	4	7	1
30	3	3	1

The cells were cultivated at a dilution rate of 0.1 h^-1. The glucose uptake flux was normalized to 100. The relative enzyme activities in the mutant were normalized by those in the wild type. Enzymes were extracted from the cultured cells and their activities were measured in vitro.

ISSN: 1752-0509