The partial least square-discriminate analysis (PLS-DA) score scatter plot of the first 2 components for WT and 2 mutants, mto1 and tt4. Dotted circles represent individual plant samples. This PLS-DA analysis represents the differentiation of 53 samples (WT, n = 17; mto1, n = 16 [13 samples from biological replicates and 3 samples from analytical replicates]; and tt4, n = 20). The symbols correspond as follows: red square, wild-type (WT); blue diamond, methionine-over accumulation 1 (mto1); green circle, transparent testa4 (tt4). The PLS-DA model shows 3 significant components according to cross-validation. The explained variation in the X-matrix (R2X) and the Y-matrix (R2Y) is 0.60 and 0.86, respectively, and the predictive ability according to 7-fold cross-validation (Q2Y) is 0.80.