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Figure 4 | BMC Systems Biology

Figure 4

From: Synthetic tetracycline-inducible regulatory networks: computer-aided design of dynamic phenotypes

Figure 4

Dynamical behavior of Network II. (A) Average number of GFP molecules in the absence of Tc (WT w/o Tc, blue line) and when 2000 molecules (WT/w 2000 Tc, green line) or 5000 molecules (WT/w 5000 Tc, red line) of Tc are added into the medium at time 2 × 104 sec, using wild-type kinetics. (B) Average number of GFP molecules when 2000 molecules of Tc are added into the medium at time 2 × 104 sec, using wild-type kinetics (WT/w 2000 Tc, blue line), a 10 fold (Des 2.1/w 2000 Tc, green line) and a 50 fold (Des 2.2/w 2000 Tc, red line) increase in the dissociation constant of both TetR and induced Tet-ON from TetO of the gene encoding Tet-ON. (C) Average number of GFP molecules when 2000 molecules of Tc are added into the medium at time 2 × 104 sec, using wild-type kinetics (WT/w 2000 Tc, blue line). All other plots have a 10 fold increase in the dissociation constant of both TetR and induced Tet-ON from TetO of the gene encoding Tet-ON, but differ in a 5 fold (Des 2.3/w 2000 Tc, green line) and 20 fold (Des 2.4/w 2000 Tc, red line) increase in the dissociation constant of both TetR and induced Tet-ON from TetO of the gene encoding TetR. (D) Average number of GFP molecules when 2000 molecules of Tc are added into the medium at time 2 × 104 sec, using wild-type kinetics (WT/w 2000 Tc, blue line). All other plots have mutated TetR variants that do not bind Tc and show a decreased binding affinity for TetO (20 fold decrease in the dissociation constant), but differ in the half-life of Tet-ON, 40 min (Des 2.5/w 2000 Tc, green line) and 24 hr (Des 2.6/w 2000 Tc, red line).

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