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Figure 5 | BMC Systems Biology

Figure 5

From: Synthetic tetracycline-inducible regulatory networks: computer-aided design of dynamic phenotypes

Figure 5

Dynamical behavior of Network III. (A) Average number of GFP molecules in the absence of Tc (WT w/o Tc, blue line) and when 2000 molecules (WT/w 2000 Tc, green line) or 5000 molecules (WT/w 5000 Tc, red line) of Tc are added into the medium at time 2 × 104 sec, using wild-type kinetics. (B) Average number of GFP molecules when 2000 molecules of Tc are added into the medium at time 2 × 104 sec, using wild-type kinetics (WT/w 2000 Tc, blue line), a 10 fold (Des 3.1/w 2000 Tc, green line) and a 50 fold (Des 3.2/w 2000 Tc, red line) increase in the dissociation constant of TetR for all TetO (mutated TetR variant). (C) Average number of GFP molecules when 2000 molecules of Tc are added into the medium at time 2 × 104 sec, using wild-type kinetics (WT/w 2000 Tc, blue line), a 5 fold (Des 3.3/w 2000 Tc, green line) and a 20 fold (Des 3.4/w 2000 Tc, red line) increase in the dissociation constant of Tet-OFF for all TetO (mutated Tet-OFF variant). (D) Average number of GFP molecules when 2000 molecules of Tc are added into the medium at time 2 × 104 sec, using for all simulations a mutated TetR variant with 10 fold (Des 3.1/w 2000 Tc, blue line) increase in the dissociation constant plus a tripled (Des 3.5/w 2000 Tc, green line) and a quadrupled (Des 3.6/w 2000 Tc, red line) half-life for both Tet-ON and TetR.

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