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Figure 4 | BMC Systems Biology

Figure 4

From: Modelling phagosomal lipid networks that regulate actin assembly

Figure 4

Experimental and model values for 32P incorporation in PIP and PIP2. A. Phagosomal preparations were incubated with γ32P.ATP at 20°C for different periods of time in the presence of different concentrations of unlabelled ATP. Phospholipids were extracted with chloroform:methanol and resolved by TLC. The radioactivity incorporated in PIP and PIP2 was measured and calibrated with a γ32P.ATP standard. B. Left panel. Phagosomal preparations were pre-incubated with 0.2 mM cold ATP at 20°C for 0, 15, 30 or 60 min. Afterwards, γ32P.ATP was added and the samples were incubated for 0, 1, 2, and 4 min. Right panel. Phagosomal preparations were incubated with γ32P.ATP in the presence of 50 μM unlabelled ATP for 10 min at 20°C. Apyrase (0 or 1 U/ml) was then added to samples (arrow) and the incubation continued for 0, 5, 10, or 22 min. Radiolabelled PIP was measured as in A. For A and B, experimental data are plotted as symbols; the model predictions as solid lines having the same colours than the corresponding experimental observations. C. Model predicted variation of ATP and phospholipids at two concentration of ATP: 0.2 mM (left panel) and 3.2 mM (right panel).

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