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Table 1 Differentially expressed proteins.

From: Comprehensive proteomic analysis of bovine spermatozoa of varying fertility rates and identification of biomarkers associated with fertility

Accesion

Name

Peptides

∑Xcorr

P-value

Regulation

  

HF

LF

HF

LF

  

115496714

Actin-like 7B

16

7

60.06

42.11

0.02243

up

77736067

Acyl-CoA thioesterase 9

14

4

39.23

16.48

2.11E-04

up

41386786

A-kinase anchor protein 4

679

581

2581.8

2424.3

0.001694

up

30794280

Albumin

7

1

27.09

5.24

1.49E-04

up

60302887

Aldose reductase

1

3

3.03

12.82

0.03293

down

27807289

Annexin A2

4

10

29.17

26.46

0.04155

down

84490369

ATP synthase, H+ transporting, mitochondrial

16

8

48.01

31.88

0.0333

up

28603752

ATP synthase, H+ transporting, mitochondrial F0

7

1

18.99

7.14

0.005907

up

28461221

ATP synthase, H+ transporting, mitochondrial F1 complex

201

174

765.55

700.71

0.006727

up

28461251

ATPase inhibitory factor 1 precursor

18

10

45.95

30.03

0.01581

up

27807145

Casein kinase 2, alpha prime polypeptide

3

0

11.25

0

5.01E-04

up

60101831

Cytochrome c oxidase subunit III

5

4

17.45

11.47

0.01008

up

84000107

Glycoprotein (transmembrane) nmb

0

3

6.9

8.98

0.0133

down

84000035

Hypothetical protein LOC504736

3

1

8.66

2.04

0.03204

up

115497288

Hypothetical protein LOC506544

4

7

13.54

18.39

0.04931

down

78369248

Hypothetical protein LOC509274

18

10

77.94

51.72

0.01217

up

115496338

Hypothetical protein LOC516024

27

22

109.76

67.93

1.54E-05

up

115495377

Hypothetical protein LOC520260

44

58

201.9

232.07

0.04396

down

114052468

Hypothetical protein LOC532785

8

19

32.19

55.25

0.003313

down

115496742

Hypothetical protein LOC534599

27

11

88.93

61.57

0.02739

up

84000301

Hypothetical protein LOC534927

9

3

32.16

14.67

0.01593

up

115495951

Hypothetical protein LOC540767

20

12

62.88

41.8

0.004421

up

94966950

Hypothetical protein LOC614199

4

0

10.84

1.66

0.007311

up

84000391

Hypothetical protein LOC615316

11

5

35.17

24.34

0.02524

up

115497750

Hypothetical protein LOC617117

19

12

58.06

44.14

0.03432

up

116004271

Hypothetical protein LOC767959

4

0

12.9

3.03

0.04429

up

27805989

Lysyl oxidase-like 4

1

3

1.59

7.16

0.03124

down

27806307

Mitochondrial ATP synthase, O subunit

13

8

51.79

34.78

0.03731

up

28461275

NADH dehydrogenase (ubiquinone) 1 alpha subcomplex

13

3

48.9

27.96

0.05046

up

28461255

NADH dehydrogenase (ubiquinone) 1 beta subcomplex

2

4

3.67

13.56

0.01051

down

62751972

Potassium voltage-gated channel shaker-related

3

0

5.86

0

1.21E-04

up

119891540

PREDICTED: glutathione S-transferase kappa 1

3

3

10.97

8.47

0.01147

up

119887606

PREDICTED: hypothetical protein

1

3

2.03

8.02

0.04382

down

119903031

PREDICTED: hypothetical protein

5

15

25.57

46.02

2.23E-04

down

119908822

PREDICTED: hypothetical protein

3

1

7.23

1.65

0.03051

up

119888977

PREDICTED: hypothetical protein

11

3

37.09

22.02

0.03232

up

119905186

PREDICTED: hypothetical protein

0

3

0

6.3

1.90E-05

down

76661674

PREDICTED: hypothetical protein

1

4

2.22

9.87

0.01595

down

119901076

PREDICTED: hypothetical protein

1

5

9.84

11.22

0.04157

down

119918378

PREDICTED: hypothetical protein

3

15

16.31

36.68

5.24E-04

down

61843441

PREDICTED: hypothetical protein

0

4

0

9.99

4.37E-05

down

119901737

PREDICTED: hypothetical protein

4

6

20.4

18.24

0.02245

down

119904572

PREDICTED: hypothetical protein

3

0

6.15

4.95

0.01124

up

119884876

PREDICTED: hypothetical protein

3

2

9.65

2.72

0.03549

up

76631114

PREDICTED: hypothetical protein

35

15

106.7

81.25

0.02224

up

119923822

PREDICTED: hypothetical protein

63

51

205.06

170.94

0.001919

up

76644873

PREDICTED: hypothetical protein isoform 2

3

0

7.78

1.83

0.03381

up

76645752

PREDICTED: hypothetical protein isoform 4

1

4

1.62

9.68

0.003268

down

119912558

PREDICTED: hypothetical protein isoform 6

4

0

10.87

0

7.37E-12

up

119893872

PREDICTED: hypothetical protein LOC535130

5

1

14.93

12.61

0.03256

up

76687954

PREDICTED: hypothetical protein, partial

3

0

7.7

1.95

0.04264

up

119925886

PREDICTED: hypothetical protein, partial

3

0

6.76

5.69

0.01623

up

119895251

PREDICTED: profilin 3

2

4

12.89

19.46

0.01716

down

119922439

PREDICTED: similar to 1700016M24Rik protein

3

0

8.17

0

1.04E-04

up

119879571

PREDICTED: similar to AAT1-alpha

22

12

69.77

50.74

0.01412

up

119912554

PREDICTED: similar to Ace protein

15

4

55.74

20.29

8.69E-06

up

61878077

PREDICTED: similar to Actin-related protein T1

7

2

16.82

7.44

0.02775

up

119928361

PREDICTED: similar to ADAM metallopeptidase

7

2

23.85

10.3

0.01155

up

119913547

PREDICTED: similar to ADAM metallopeptidase with thrombospondin type 1 motif, 17 preproprotein

5

0

12.58

0

1.24E-07

up

119903267

PREDICTED: similar to ALMS1 protein

4

0

10.2

2.89

0.02294

up

119892487

PREDICTED: similar to Ankyrin repeat domain-containing

1

3

1.88

7.82

0.03473

down

76657564

PREDICTED: similar to calmodulin

10

5

23.53

16.83

0.04331

up

119901005

PREDICTED: similar to centrosomal protein 110kD

3

0

8.38

2.06

0.03921

up

119893858

PREDICTED: similar to chromosome 13 open reading

0

3

0

6.84

4.35E-04

down

61814552

PREDICTED: similar to Cytochrome c oxidase subunit

13

10

41.99

47.17

0.01639

up

119904416

PREDICTED: similar to diaphanous homolog 3

0

3

0

7.64

3.85E-04

down

119915202

PREDICTED: similar to DNAH8, partial

12

3

33.72

16.39

0.001961

up

119927503

PREDICTED: similar to DNAH8, partial

6

1

22.2

8.36

0.04519

up

119911633

PREDICTED: similar to EF-hand calcium binding domain 5

0

3

0

7.52

3.23E-05

down

119888835

PREDICTED: similar to EPH receptor A8

4

0

9.46

1.9

0.009866

up

119895747

PREDICTED: similar to FAT tumor suppressor 2

12

3

28.56

32.84

1.15E-04

up

119919673

PREDICTED: similar to ferritin L subunit isoform

1

4

0.57

10.42

3.74E-05

down

119909426

PREDICTED: similar to fertilin alpha

12

27

63.45

89.9

0.003791

down

119919953

PREDICTED: similar to filamin

0

3

0

6.94

4.54E-04

down

76662361

PREDICTED: similar to GFHL3075 isoform 3

3

0

8.3

7.63

0.01657

up

61820991

PREDICTED: similar to GK2 protein

16

9

54.86

31.79

0.01383

up

119901324

PREDICTED: similar to HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1 isoform 1

2

3

6.09

6.74

0.01418

down

119895512

PREDICTED: similar to HIST1H4I protein

3

0

6.36

1.06

0.007699

up

119915532

PREDICTED: similar to histone H2b-616

4

10

17.41

34.08

0.005828

down

76613952

PREDICTED: similar to histone H4

2

2

7.48

5.88

0.02561

down

76642199

PREDICTED: similar to Izumo sperm-egg fusion 1

39

24

133.67

99.29

0.005314

up

119890207

PREDICTED: similar to KIAA0191

18

26

70.99

64.29

0.01024

down

119891377

PREDICTED: similar to KIAA0225 isoform 1

1

4

1.01

9.43

5.50E-04

down

119890395

PREDICTED: similar to KIAA0467 protein

0

3

0

7.91

3.74E-06

down

119902048

PREDICTED: similar to KIAA1305 protein

0

3

0

7.91

0.001712

down

119906772

PREDICTED: similar to KIAA1429 protein isoform

0

3

0

6.98

4.55E-04

down

119912552

PREDICTED: similar to KIAA1636 protein

3

0

7.44

0

1.53E-04

up

119891313

PREDICTED: similar to KIAA1793 protein

12

8

52.91

37.11

0.0228

up

119909205

PREDICTED: similar to KIAA2017 protein isoform

6

0

17.51

6.69

0.01123

up

76664109

PREDICTED: similar to LOC505732 protein

4

6

14.08

12.97

0.01832

down

76641602

PREDICTED: similar to LOC507431 protein isoform

10

7

31.93

36.41

0.03413

up

119902010

PREDICTED: similar to LOC512571 protein

47

34

189.9

155.47

0.03649

up

119905900

PREDICTED: similar to NDRG3

0

3

0

7.3

1.80E-04

down

76612380

PREDICTED: similar to nestin

0

3

0

6.85

1.25E-04

down

119911939

PREDICTED: similar to netrin-1

2

4

2.84

8.74

0.01328

down

119894490

PREDICTED: similar to obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF, partial

1

3

1.45

7.16

0.03376

down

119905455

PREDICTED: similar to Pitrilysin metallopeptidase

1

6

2.49

22.6

3.23E-05

down

76618065

PREDICTED: similar to Pou6f1 protein

4

1

15.92

11.31

0.006931

up

119894859

PREDICTED: similar to Protein KIAA1543 isoform

1

3

1.36

8.01

0.008488

down

119893105

PREDICTED: similar to protein kinase A binding protein

135

113

480.21

392.89

2.35E-05

up

76627105

PREDICTED: similar to RAB2B, member RAS oncogene

7

16

29.46

52.58

0.004367

down

119912290

PREDICTED: similar to RIKEN cDNA 4121402D02

3

0

7.29

0

2.00E-07

up

119914167

PREDICTED: similar to RIKEN cDNA A530050D06 gene

6

8

12.57

21.82

0.007372

down

119903563

PREDICTED: similar to RNA polymerase I polypept

3

6

7.15

15.59

0.03155

down

119910233

PREDICTED: similar to sca1

3

0

7.43

1.65

0.0301

up

119916698

PREDICTED: similar to Septin 12

0

3

0

8.2

2.26E-07

down

119903556

PREDICTED: similar to sulfotransferase K1

0

3

0

6.79

1.49E-04

down

119902145

PREDICTED: similar to telomerase-associated protein

3

0

8.27

0

8.45E-06

up

119914302

PREDICTED: similar to trans-Golgi p230

4

0

8.82

2.85

0.01983

up

119917225

PREDICTED: similar to TRRAP protein

2

5

2.92

11.86

0.002132

down

119917582

PREDICTED: similar to TUBA

3

1

9.42

3.39

0.03158

up

119912117

PREDICTED: similar to Tumor necrosis factor receptor

7

0

17.77

0

6.66E-12

up

119903686

PREDICTED: similar to ubiquitin specific protease 34 isoform1

1

4

2.31

9.99

0.01666

down

77736091

Prohibitin

7

3

22.59

9.08

0.03313

up

114052901

Rhabdoid tumor deletion region gene 1

10

2

31.71

15.41

0.009593

up

84000339

Sperm associated antigen 6

4

1

13.76

2.2

0.02495

up

87196516

Sperm mitochondria-associated cysteine-rich protein

3

0

9.82

3.51

0.03455

up

115495195

Tektin 1

27

19

107.5

76.3

0.0155

up

84000201

Transmembrane protein 5

3

1

6.27

6.97

0.004588

up

61888856

Triosephosphate isomerase

40

26

158.4

122.55

0.005981

up

27807143

Ubiquinol-cytochrome c reductase core protein II

7

2

33.24

16.14

0.02472

up

  1. List of differentially expressed proteins in high fertility (HF) group spermatozoa when compared to low fertility group. (LF) spermatozoa In this table we provided the information about number of peptides, Sequest cross correlation (∑Xcorr) score and P value for each protein in high and low fertility group spermatozoa respectively.