Table 1 Reaction mechanism and protein abundance for glycolytic pathways of E. coli
From: Extraction of elementary rate constants from global network analysis of E. coli central metabolism
Enzyme | Abundance a (molecules/cell) | Concentration b (μM) | Kinetic mechanism | Elementary reactions |
|---|---|---|---|---|
Phosphogluco -isomerase (pgi) | 1099 | 2.7 | Reversible Uni Uni [51] | G6P + EPGI → EPGI-G6P |
|  |  |  |  | EPGI-G6P → G6P + EPGI |
|  |  |  |  | EPGI-G6P → F6P +EPGI |
|  |  |  |  | F6P + EPGI → EPGI-G6P |
Phosphofructo -kinase (pfkA) | 3287 | 8.2 | Allosteric regulation and ordered sequential mechanism [39] [52] | ATP + EPFKATR → EPFKAR-ATP |
|  |  |  |  | EPFKAR-ATP → ATP + EPFKATR |
|  |  |  |  | F6P + EPFKAR-ATP → EPFKAR-ATP-F6P |
|  |  |  |  | EPFKAR-ATP-F6P → F6P + EPFKAR-ATP |
|  |  |  |  | EPFKAR-ATP-F6P → FDP + EPFKAR-ADP |
|  |  |  |  | EPFKAR-ADP → ADP + EPFKATR |
Fructose-bisphosphate aldolase (fba) | 16326 | 40.5 | Ordered Uni Bi [51] | FDP + EFBA → EFBA-FDP |
|  |  |  |  | EFBA-FDP → FDP + EFBA |
|  |  |  |  | EFBA-FDP → GAP + EFBA-DHAP |
|  |  |  |  | GAP + EFBA-DHAP → EFBA-FDP |
|  |  |  |  | EFBA-DHAP → DHAP + EFBA |
|  |  |  |  | DHAP + EFBA → EFBA-DHAP |
Triosephosphate isomerase (tpiA) | 9106 | 22.6 | Reversible Uni Uni [51] | DHAP + ETPIA → ETPIA-DHAP |
|  |  |  |  | ETPIA-DHAP → DHAP + ETPIA |
|  |  |  |  | ETPIA-DHAP → GAP + ETPIA |
|  |  |  |  | GAP + ETPIA → ETPIA-DHAP |
Glyceraldehyde 3-phosphate dehydrogenase (gapA) | 49091 | 121.7 | Ordered sequential mechanism [37] | NAD + EGAP → EGAP-NAD |
|  |  |  |  | EGAP-NAD → NAD + EGAP |
|  |  |  |  | GAP + EGAP-NAD → EGAP-NAD-GAP |
|  |  |  |  | EGAP-NAD-GAP → GAP + EGAP-NAD |
|  |  |  |  | EGAP-NAD-GAP → PGP + EGAP-NADH |
|  |  |  |  | PGP + EGAP-NADH → EGAP-NAD-GAP |
|  |  |  |  | EGAP-NADH → NADH + EGAP |
|  |  |  |  | NADH + EGAP → EGAP-NADH |
Phosphoglycerate kinase (pgk) | 14682 | 36.4 | Ordered sequential mechanism [53] | PGP + EPGK → EPGK-PGP |
|  |  |  |  | EPGK-PGP → PGP + EPGK |
|  |  |  |  | ADP + EPGK-PGP → EPGK-PGP-ADP |
|  |  |  |  | EPGK-PGP-ADP → ADP + EPGK-PGP |
|  |  |  |  | EPGK-PGP-ADP → ATP + EPGK-PG3 |
|  |  |  |  | ATP + EPGK-PG3 → EPGK-PGP-ADP |
|  |  |  |  | EPGK-PG3 → PG3 + EPGK |
|  |  |  |  | PG3 + EPGK → EPGK-PG3 |
Phosphoglycerate mutase (pgm) | 966 | 2.4 | Reversible Uni Uni [21] | PG3 + EPGM → EPGM-PG3 |
|  |  |  |  | EPGM-PG3 → PG3 + EPGM |
|  |  |  |  | EPGM-PG3 → PG2 + EPGM |
|  |  |  |  | PG2 + EPGM → EPGM-PG3 |
Enolase (eno) | 11283 | 28.0 | Reversible Uni Uni [21] | PG2 + EENO → EENO-PG2 |
|  |  |  |  | EENO-PG2 → PG2 + EENO |
|  |  |  |  | EENO-PG2 → PEP + EENO |
|  |  |  |  | PEP + EENO → EENO-PG2 |
Pyruvate kinase (pykF) b | 500 | 1.2 | Allosteric regulation and ordered sequential mechanism [24, 39] | PEP + EPYKFTR → EPYKFR-PEP |
|  |  |  |  | EPYKFR-PEP → PEP + EPYKFTR |
|  |  |  |  | EPYKFR-PEP + ADP → EPYKFR-PEP-ADP |
|  |  |  |  | EPYKFR-PEP-ADP → EPYKFR-PEP + ADP |
|  |  |  |  | EPYKFR-PEP-ADP → PYR + EPYKFR-ATP |
|  |  |  |  | EPYKFR-ATP → ATP + EPYKFTR |