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Table 1 Reaction mechanism and protein abundance for glycolytic pathways of E. coli

From: Extraction of elementary rate constants from global network analysis of E. coli central metabolism

Enzyme Abundance a (molecules/cell) Concentration b (μM) Kinetic mechanism Elementary reactions
Phosphogluco -isomerase (pgi) 1099 2.7 Reversible Uni Uni [51] G6P + EPGI → EPGI-G6P
     EPGI-G6P → G6P + EPGI
     EPGI-G6P → F6P +EPGI
     F6P + EPGI → EPGI-G6P
Phosphofructo -kinase (pfkA) 3287 8.2 Allosteric regulation and ordered sequential mechanism [39] [52] ATP + EPFKATR → EPFKAR-ATP
     EPFKAR-ATP → ATP + EPFKATR
     F6P + EPFKAR-ATP → EPFKAR-ATP-F6P
     EPFKAR-ATP-F6P → F6P + EPFKAR-ATP
     EPFKAR-ATP-F6P → FDP + EPFKAR-ADP
     EPFKAR-ADP → ADP + EPFKATR
Fructose-bisphosphate aldolase (fba) 16326 40.5 Ordered Uni Bi [51] FDP + EFBA → EFBA-FDP
     EFBA-FDP → FDP + EFBA
     EFBA-FDP → GAP + EFBA-DHAP
     GAP + EFBA-DHAP → EFBA-FDP
     EFBA-DHAP → DHAP + EFBA
     DHAP + EFBA → EFBA-DHAP
Triosephosphate isomerase (tpiA) 9106 22.6 Reversible Uni Uni [51] DHAP + ETPIA → ETPIA-DHAP
     ETPIA-DHAP → DHAP + ETPIA
     ETPIA-DHAP → GAP + ETPIA
     GAP + ETPIA → ETPIA-DHAP
Glyceraldehyde 3-phosphate dehydrogenase (gapA) 49091 121.7 Ordered sequential mechanism [37] NAD + EGAP → EGAP-NAD
     EGAP-NAD → NAD + EGAP
     GAP + EGAP-NAD → EGAP-NAD-GAP
     EGAP-NAD-GAP → GAP + EGAP-NAD
     EGAP-NAD-GAP → PGP + EGAP-NADH
     PGP + EGAP-NADH → EGAP-NAD-GAP
     EGAP-NADH → NADH + EGAP
     NADH + EGAP → EGAP-NADH
Phosphoglycerate kinase (pgk) 14682 36.4 Ordered sequential mechanism [53] PGP + EPGK → EPGK-PGP
     EPGK-PGP → PGP + EPGK
     ADP + EPGK-PGP → EPGK-PGP-ADP
     EPGK-PGP-ADP → ADP + EPGK-PGP
     EPGK-PGP-ADP → ATP + EPGK-PG3
     ATP + EPGK-PG3 → EPGK-PGP-ADP
     EPGK-PG3 → PG3 + EPGK
     PG3 + EPGK → EPGK-PG3
Phosphoglycerate mutase (pgm) 966 2.4 Reversible Uni Uni [21] PG3 + EPGM → EPGM-PG3
     EPGM-PG3 → PG3 + EPGM
     EPGM-PG3 → PG2 + EPGM
     PG2 + EPGM → EPGM-PG3
Enolase (eno) 11283 28.0 Reversible Uni Uni [21] PG2 + EENO → EENO-PG2
     EENO-PG2 → PG2 + EENO
     EENO-PG2 → PEP + EENO
     PEP + EENO → EENO-PG2
Pyruvate kinase (pykF) b 500 1.2 Allosteric regulation and ordered sequential mechanism [24, 39] PEP + EPYKFTR → EPYKFR-PEP
     EPYKFR-PEP → PEP + EPYKFTR
     EPYKFR-PEP + ADP → EPYKFR-PEP-ADP
     EPYKFR-PEP-ADP → EPYKFR-PEP + ADP
     EPYKFR-PEP-ADP → PYR + EPYKFR-ATP
     EPYKFR-ATP → ATP + EPYKFTR
  1. a Protein abundances are taken from mass spectrometry data [34], with the exception of the abundance for pykF which is taken from 2-D gel data [35]
  2. b Volume concentration is calculated based on E. coli cytoplasm volume equivalent to 6.7 × 10-16 L [54]