Figure 4

Coordinate events which resulted in gene regulation in response to c-Myc overexpression. 1) c-Myc putatively binds to 110 binding sites in 94 different promoters of induced genes. Binding sites (BSs) of four different transcription factors (TFs) have been shown to be overrepresented in the direct neighborhood of c-Myc binding sites. They either cooperate with or compete againstc-Myc. 2) Some of the genes induced by c-Myc overexpression code for transcription factors. These transcription factors constitute the basis of a regulatory gene network that again influences the expression of different genes. Taken collectively, 220 different promoters of induced genes possess binding sites for those induced transcription factors, matrices of which areavailable. 3) Other transcription factors which could be part of the c-Myc regulatory gene network are those which might be activated on the protein level through the overexpression of c-Myc.