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Table 4 Parameters estimation results of acetate and propionate enriched cultures for the different feeding conditions

From: Metabolic modelling of polyhydroxyalkanoate copolymers production by mixed microbial cultures

Substrate

Acetate

Propionate

Mixture

Culture enrichment

Acetate

Propionate

Acetate

Propionate

Acetate

Propionate

Number of experiments used for parameters estimation/model validation

(7/3)

(1/0)

(1/0)

(3/1)

(1/0)

(1/0)

P/O ratio, d (mol-ATP/mol-NADH2)

2.94 ± 0.16

1.08 ± 0.12

1.80 ± 0.19

0.94 ± 0.04

2.90 ± 0.27

1.78 ± 0.19

Maximum rates determined by metabolic model [C-mol/(C-mol.h)]

R 6, max

-

-

-

-

0.092 ± 0.017

0.094 ± 0.046

R 9, max

0.52 ± 0.03

0.13 ± 0.01

0.033 ± 0.005

0.053 ± 0.003

0.30 ± 0.03

0.18 ± 0.02

R 10 max

-

-

0.041 ± 0.004

0.052 ± 0.003

0.22 ± 0.02

0.13 ± 0.02

Maximum rates determined by MFA [C-mol/(C-mol.h)]

R 1 max

0.73 (0.01)

0.21 (0.02)

-

-

0.33 (0.07)

0.22 (0.05)

R 2 max

-

-

0.12 (0.02)

0.17 (0.02)

0.34 (<0.01)

0.23 (<0.01)

R 3 max

-

-

0.049 (0.005)

0.074 (0.005)

0.074 (0.018)

0.059 (0.016)

R 6 max

0.18 (<0.01)

0.07 (<0.01)

0.016 (<0.001)

0.021 (0.003)

-

-

R 8 max

0.24 (0.02)

0.11 (<0.01)

0.094 (0.006)

0.13 (0.02)

0.19 (<0.01)

0.22 (0.01)

R PHB, max

0.52 (0.03)

0.13 (0.01)

0.011 (0.003)

0.018 (0.001)

0.14 (0.02)

0.090 (0.005)

R PHV, max

-

-

0.035 (0.006)

0.086 (0.006)

0.26 (0.03)

0.22 (0.03)

R PH 2MV, max

-

-

0.026 (0.003)

-

0.12 (0.01)

-

  1. The confidence bounds in parenthesis were obtained by error propagation of the metabolic model parameters
  2. fPHA, max= 2.47 C-mmol/C-mmol [10]; a = 3.85 (dimensionless) [10]; m ATP = 0.02 mol-ATP/(C-mol.h) [39]; K S = 0.062 C-mmol/l [39]; K N = 0.56 N-mmol/l [40]