Figure 2

Metabolic net flux distributions of S. cerevisiae CEN.PK113-1A in different oxygenation conditions. Net flux distribution of S. cerevisiae CEN.PK113-1A in glucose-limited chemostat, D = 0.1 h^-1, in different oxygenation conditions: 20.9%, 2.8%, 1.0%, 0.5% and 0.0% oxygen of the chemostat inlet gas. The net fluxes are shown as relative fluxes normalised to the specific glucose uptake rate in the corresponding experiment. The fluxes for each reaction in the model corresponding to the 20.9%, 2.8%, 1.0%, 0.5% and 0.0% oxygen of the chemostat inlet gas are given from top to bottom and the flux values from replicate experiment are given from left to right. The specific glucose uptake rates corresponding to the different oxygenation conditions and the replicate experiments are given at the top of the figure. The net flux distributions were determined using fractional [U-¹³C]glucose feed and metabolic flux ratio (METAFoR) analysis to obtain additional experimental constraints to render an underdetermined metabolite mass balance system solvable. The Matlab function fmincon performing nonlinear optimisation was applied to solve the net fluxes.