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Table 1 Uptake and production rates and biomass concentration in S. cerevisiae CEN.PK113-1A chemostat cultures. Glucose uptake rate, glycerol, acetate and ethanol production rate and biomass concentration (mean ± SEM) of the 13C-labelled glucose-limited chemostat cultures (D = 0.10 h-1, pH 5.0, 30°C, 1,5 vvm gas flow) of S. cerevisiae CEN.PK113-1A used as input values in the 13C-MFA.

From: Oxygen dependence of metabolic fluxes and energy generation of Saccharomyces cerevisiae CEN.PK113-1A

  O2 provided
  20.9% 2.8% 1.0% 0.5% 0.0%
  I II I II I II I II I II
Glucose uptake rate [mmol/(g DW)-1h-1] 1.28 ± 0.04 0.87 ± 0.04 1.36 ± 0.04 1.28 ± 0.02 1.97 ± 0.02 2.13 ± 0.06 2.20 ± 0.09 2.78 ± 0.16 6.30 ± 0.25 6.58 ± 0.16
Glycerol production rate [mmol/(g DW)-1h-1] 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 1.05 ± 0.01 1.11 ± 0.03
Acetate production rate [mmol/(g DW)-1h-1] 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00 0.00 ± 0.00
Ethanol production rate [mmol/(g DW)-1h-1] 0.00 ± 0.00 0.00 ± 0.00 0.07 ± 0.00 0.10 ± 0.01 1.56 ± 0.02 2.00 ± 0.08 2.59 ± 0.09 2.91 ± 0.18 9.05 ± 0.21 9.47 ± 0.31
Biomass (g DW l-1) 5.17 ± 0.04 5.31 ± 0.12 4.61 ± 0.09 4.86 ± 0.05 3.22 ± 0.02 2.70 ± 0.03 2.21 ± 0.03 2.08 ± 0.03 1.03 ± 0.02 0.98 ± 0.02