Skip to main content

Advertisement

Table 2 Metabolic flux ratio (METAFoR) analysis results. Metabolic flux ratio (METAFoR) analysis results showing the origins of metabolic intermediates during growth of S. cerevisiae CEN.PK113-1A in glucose-limited, 13C-labelled chemostat cultures (D = 0.1 h-1) at different fractions of oxygen in the chemostat inlet gas. Values for two independent cultivations are given for each condition.

From: Oxygen dependence of metabolic fluxes and energy generation of Saccharomyces cerevisiae CEN.PK113-1A

Metabolite % fraction of total pool
  20.9% O2 2.8% O2 1.0% O2 0.5% O2 0.0% O2
  I II I II I II I II I II
Pep from pentose phosphates (ub)a 30 ± 9 34 ± 11 19 ± 6 20 ± 7 15 ± 6 19 ± 7 10 ± 7 6 ± 9 4 ± 4 4 ± 5
P5P from G3P and S7P (transketolase reaction) 51 ± 3 56 ± 6 64 ± 5 63 ± 4 82 ± 3 77 ± 3 74 ± 6 79 ± 4 86 ± 3 86 ± 5
P5P from E4P (transketolase and transaldolase) 34 ± 2 35 ± 2 27 ± 2 25 ± 2 28 ± 2 24 ± 2 26 ± 2 38 ± 2 14 ± 2 15 ± 2
Ser from Gly and C1-unit 62 ± 4 61 ± 4 61 ± 4 61 ± 4 63 ± 3 62 ± 3 62 ± 4 58 ± 3 57 ± 3 58 ± 3
Gly from CO2 and C1-unit 4 ± 4 3 ± 3 5 ± 3 6 ± 3 4 ± 3 4 ± 3 0 ± 4 4 ± 3 4 ± 3 2 ± 3
Pep from Oaacyt (PEPck) 4 ± 7 7 ± 8 3 ± 6 1 ± 6 2 ± 10 7 ± 10 6 ± 12 0 ± 14 nd nd
Oaamit from Pep 30 ± 2 31 ± 2 30 ± 2 29 ± 2 34 ± 2 38 ± 2 48 ± 2 57 ± 2 100 ± 2 100 ± 2
Oaamit from Oaacyt 50 ± 3 55 ± 4 52 ± 4 54 ± 3 45 ± 2 51 ± 2 60 ± 3 69 ± 2 99 ± 2 99 ± 2
Oaacyt from Pep 43 ± 2 37 ± 3 39 ± 3 35 ± 2 61 ± 3 57 ± 3 62 ± 4 59 ± 4 nd nd
Oaacyt reversibly converted to fumarate 10 ± 7 18 ± 17 19 ± 7 17 ± 10 6 ± 5 10 ± 9 8 ± 5 14 ± 4 18 ± 7 21 ± 3
Oaamit reversibly converted to fumarate 64 ± 15 77 ± 17 71 ± 15 61 ± 14 60 ± 13 60 ± 13 62 ± 11 70 ± 8 29 ± 4 27 ± 4
Pyrmit from malate (ub)a 3 ± 3 2 ± 6 4 ± 4 4 ± 3 0 ± 4 0 ± 4 ndb nd nd nd
Pyrmit from malate (lb)a 2 ± 2 1 ± 4 3 ± 3 3 ± 2 0 ± 2 0 ± 2 nd nd nd nd
  1. a ub upper bound, lb lower bound for the fraction of total pool, see Methods for details
  2. b nd not determined, see Methods for details