Table 2 Metabolic flux ratio (METAFoR) analysis results. Metabolic flux ratio (METAFoR) analysis results showing the origins of metabolic intermediates during growth of S. cerevisiae CEN.PK113-1A in glucose-limited, ¹³C-labelled chemostat cultures (D = 0.1 h^-1) at different fractions of oxygen in the chemostat inlet gas. Values for two independent cultivations are given for each condition.

Pep from pentose phosphates (ub)^a

30 ± 9

34 ± 11

19 ± 6

20 ± 7

15 ± 6

19 ± 7

10 ± 7

6 ± 9

4 ± 4

4 ± 5

P5P from G3P and S7P (transketolase reaction)

51 ± 3

56 ± 6

64 ± 5

63 ± 4

82 ± 3

77 ± 3

74 ± 6

79 ± 4

86 ± 3

86 ± 5

P5P from E4P (transketolase and transaldolase)

34 ± 2

35 ± 2

27 ± 2

25 ± 2

28 ± 2

24 ± 2

26 ± 2

38 ± 2

14 ± 2

15 ± 2

Ser from Gly and C1-unit

62 ± 4

61 ± 4

61 ± 4

61 ± 4

63 ± 3

62 ± 3

62 ± 4

58 ± 3

57 ± 3

58 ± 3

Gly from CO₂ and C1-unit

4 ± 4

3 ± 3

5 ± 3

6 ± 3

4 ± 3

4 ± 3

0 ± 4

4 ± 3

4 ± 3

2 ± 3

Pep from Oaa_cyt (PEPck)

4 ± 7

7 ± 8

3 ± 6

1 ± 6

2 ± 10

7 ± 10

6 ± 12

0 ± 14

nd

nd

Oaa_mit from Pep

30 ± 2

31 ± 2

30 ± 2

29 ± 2

34 ± 2

38 ± 2

48 ± 2

57 ± 2

100 ± 2

100 ± 2

Oaa_mit from Oaa_cyt

50 ± 3

55 ± 4

52 ± 4

54 ± 3

45 ± 2

51 ± 2

60 ± 3

69 ± 2

99 ± 2

99 ± 2

Oaa_cyt from Pep

43 ± 2

37 ± 3

39 ± 3

35 ± 2

61 ± 3

57 ± 3

62 ± 4

59 ± 4

nd

nd

Oaa_cyt reversibly converted to fumarate

10 ± 7

18 ± 17

19 ± 7

17 ± 10

6 ± 5

10 ± 9

8 ± 5

14 ± 4

18 ± 7

21 ± 3

Oaa_mit reversibly converted to fumarate

64 ± 15

77 ± 17

71 ± 15

61 ± 14

60 ± 13

60 ± 13

62 ± 11

70 ± 8

29 ± 4

27 ± 4

Pyr_mit from malate (ub)^a

3 ± 3

2 ± 6

4 ± 4

4 ± 3

0 ± 4

0 ± 4

nd^b

nd

nd

nd

Pyr_mit from malate (lb)^a

2 ± 2

1 ± 4

3 ± 3

3 ± 2

0 ± 2

0 ± 2

nd

nd

nd

nd