Table 2 Kinetic parameters of VEGF in the healthy tissue (human vastus lateralis muscle)

VEGF binding to VEGFR1

k_on

3 10⁷

M^-1 s^-1

4.8 10^-1

(pmol/cm³ tissue)^-1 s^-1

k_off

10^-3

s^-1

K_d

33

pM

2.0 10^-3

pmol/cm³ tissue

VEGF binding to VEGFR2

k_on

10⁷

M^-1 s^-1

1.6 10^-1

(pmol/cm³ tissue)^-1 s^-1

k_off

10^-3

s^-1

K_d

100

pM

6.4 10^-3

pmol/cm³ tissue

VEGF165 binding to NRP1

k_on

3.2 10⁶

M^-1 s^-1

5.1 10^-2

(pmol/cm³ tissue)^-1 s^-1

k_off

10^-3

s^-1

K_d

312

pM

2.0 10^-2

pmol/cm³ tissue

VEGF165 binding to GAGs

k_on

4.2 10⁵

M^-1 s^-1

6.7 10^-3

(pmol/cm³ tissue)^-1 s^-1

k_off

10^-2

s^-1

K_d

24

nM

1.5

pmol/cm³ tissue

Coupling of NRP1 & VEGFR2

k_{cV165R2, N1}

3.1 10¹³

(mol/cm²)^-1 s^-1

2.8 10^-1

(pmol/cm³ tissue)^-1 s^-1

k_offV165R2,N1

10^-3

s^-1

k_cV165N1,R2

10¹⁴

(mol/cm²)^-1 s^-1

9.2 10^-1

(pmol/cm³ tissue)^-1 s^-1

k_offV165N1,R2

10^-3

s^-1

VEGFR1 coupling to NRP1

k_cR1,N1

10¹⁴

(mol/cm²)^-1 s^-1

9.2 10^-1

(pmol/cm³ tissue)^-1 s^-1

k_dissocR1,N

10^-2

s^-1

VEGFR internalization

k_int,R

2.8 10^-4

s^-1

k_int,C

2.8 10^-4

s^-1