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Table 3 VEGF concentration and receptor densities for the healthy tissue (human vastus lateralis)

From: A compartment model of VEGF distribution in blood, healthy and diseased tissues

  Measured parameter Tissue model
  Value Unit Value Unit
Free VEGF concentration
Human vastus lateralis, rest 1 pM 6.2 10-5 pmol/cm3 tissue
Total VEGF tissue concentration
Human vastus lateralis, rest 1–2 pg/μ g protein 3.4–6.9 pmol/cm3 tissue
VEGFR1 tissue concentration
Human vastus lateralis, rest 1.6–1.8 pg/μ g protein 1.1–1.2 pmol/cm3 tissue
    60,000–68,000 #/EC
VEGFR2 tissue concentration
Human vastus lateralis, rest 0.33–0.5 pg/μ g protein 0.24–0.34 pmol/cm3 tissue
    13,000–19,000 #/EC
NRP1 tissue concentration
    0.018–1.8 pmol/cm3 tissue
    1,000–100,000 #/EC
ECM binding site density
ECM 0.75 μ M 46 pmol/cm3 tissue
Vessel BM 13 μ M 1 pmol/cm3 tissue
Myocyte BM 13 μ M 4 pmol/cm3 tissue
  1. The conversion of receptor densities to tissue concentrations is based on the relationship mentioned in table 2, and the surface area of an endothelial cell, 1000 μ m2. VEGF concentration is normalized based on the entire interstitial space, since it diffuses throughout: 6.2 107 (pmol/cm3 tissue)/M (here, M = moles/liter interstitial space). VEGF binding sites in the ECM and BMs are based on those volumes: 6.2 107 (pmol/cm3 tissue)/M(ECM), 5.7 104 (pmol/cm3 tissue)/M(EBM), 3.1 105 (pmol/cm3 tissue)/M (MBM). For example, M(EBM) = moles/liter endothelial basement membrane. Conversions from pg/mg protein are based on 155 mg protein/g of tissue and 45 kDa VEGF, 210 kDa VEGFR1, 240 kDa VEGFR2 [6].