A. Pie chart showing the distribution of i JN746's intracellular reactions over the different subsystems. The number of reactions per subsystem is shown and subsystems of high importance were highlighted in bold. B. Heat map of the confidence score of the different subsystems in i JN746. The 4 rows in the map represent the different confidence score (from left to right: 4, 3, 2, 1). The various colors correspond to the percentage of subsystems reactions that have the corresponding confidence score (red = 100%, blue = 0%). The confidence level was based on a scale from 1 to 4. A level, or score, of 4 corresponds to biochemical evidence for a gene product and its reaction(s); 3 represents physiological, genetic, or proteomic evidence; 2 corresponds to only sequence-based evidence for a gene product and its reaction(s); and finally a score of 1 reflects that the reaction had to be included for model functionality (e. g., production of biomass precursor).