A genome-scale metabolic reconstruction of Pseudomonas putida KT2440: i JN746 as a cell factory

BMC Systems Biology

Table 3 Comparison of growth performance of the in silico strain i JN746 and KT2442.

Strain	Carbon source	μmax(h^-1) iJN746	μmax(h^-1) KT2442	Carbon Uptake rate (mmol gDW/h)	O₂ Uptake rate (mmol gDW/h)
i JN746/KT2442	Glucose	0.751	0.56a	6.3a	15.34d
i JN746/KT2442	Toluene	0.421	0.72b	11.9b	18.5c
i JN746/KT2442	Toluene	0.476	0.72	11.9	20.93d
i JN746/KT2442	Toluene	0.7255	0.72	11.9	33
i JN746/KT2442	Toluene	1.262	0.72	11.9	∞

Comparison of growth performance of the in silico strain i JN746 and KT2442. The in silico growth rate was calculated in i M9 minimal medium plus glucose or toluene. Due to candidate oxygen limited growth in toluene, the in silico growth rate was calculated under different oxygen uptake rates In addition i JN746 growth in toluene as only carbon source was simulated at different oxygen uptake rates. ^a from [25]; ^b from[26]; ^c from [78], and ^d experimentally determined in this study.

ISSN: 1752-0509