Figure 7
Analysis of nucleotide exchange activity for different translation factors. A, nucleotide exchange rates must match protein production rates (for translation initiation and termination factors) or elongation rates (for elongation factors) due to the cyclical nature of the relevant reactions. B, kinetic models used for the analyses in panels C-F. Nucleotide exchange on eIF2 was calculated from published K M (7.4 × 10-8 M) and k cat (43 s-1) values according to the Michaelis-Menten equation. All other reactions were implemented as kinetic computer models and analysed for maximum nucleotide exchange rates. Rate constants for the exchange reactions on eIF5B are from [44], on eRF1:eRF3 complexes from [38], and on eEF1A from [8]. C-F results of simulations of nucleotide exchange reactions. In all four panels, areas shaded in red indicate the exchange activity required to match the predicted translational activity, within the 90% confidence limits for these predictions. Nucleotide exchange rates are plotted as a function of the relevant factor:GDP complex concentration (black lines), mean intracellular concentrations of total factor are indicated by green vertical lines for reference (except for eEF1A where the concentration is from a single study, see main text for explanation).