Figure 5
From: Simulating in vitro transcriptional response of zinc homeostasis system in Escherichia coli
Comparison of simulated results and experimental data (II). (a) ZntR transcription assay with Zn(II) or without Zn(II). Red dots for the case of with Zn(II) and green dots for the case of without Zn(II) are reconstructed from the reported data in the original figure (Fig. 6B in [29]) using image analysis. Error bars indicate a standard deviation both above and below the average values of two separate experiments. The purple line and the green line are the corresponding simulated final concentrations (in nM) of mRNA of ZntA (Mz(t = td 0+ t d )) in the cases of parameter Zn tot = 10 μM and Zn tot = 0 as a function of the logarithm of [ZntR] total (i.e., 2*Px tot ), respectively. (b) ZntR transcription assay with varying total zinc concentration. The black dots are reconstructed from the reported data in the original figure (Fig. 6C in [29]) using image analysis. The black curve is the simulated final concentration (in nM) of mRNA of ZntA (Mz(t = td 0+ t d )) as a function of the logarithm of parameter Zn tot (also denoted as [Zn] total ).