Figure 8

Root locus plots of RAF1 to MKNK2 SISO system. Fig. 8a Root locus plot of RAF1 to MKNK2 system perturbed by wild type Vpr with negative feedback where a large portion of the locus can be seen within the unit circle. Fig. 8b Root locus plot of RAF1 to MKNK2 system perturbed by R73A mutant Vpr with negative feedback showing very good stability margins. Fig. 8c Root locus plot of RAF1 to MKNK2 system perturbed by R80A mutant Vpr with negative feedback, where there is basically no stability margin due to the two loci on the real axis. Fig. 8d Root locus plot of RAF1 to MKNK2 system perturbed by R80A mutant Vpr with positive feedback and small stability margins.