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Figure 1 | BMC Systems Biology

Figure 1

From: Modeling ERBB receptor-regulated G1/S transition to find novel targets for de novo trastuzumab resistance

Figure 1

Characterization of breast cell lines for trastuzumab resistance/sensitivity. A. qRT-PCR to determine ERBB receptor family expression at mRNA level in MCF-12A normal breast epithelial cells and in five breast carcinoma cells. B. Western blots showing the expression level of ERBB family receptors. HCC1954 cells express high levels of ERBB1 and ERBB2 receptors, but low level of ERBB3 and no ERBB4. β-actin was used as loading control. C. WST-1 cell viability assay to assess the resistance of breast carcinoma cells to trastuzumab (100 nM). Compared to SK-BR-3 cells, with high level of ERBB2 receptors, HCC1954 cells are resistant to trastuzumab (100 nM) over 4 days. D. Verification of resistance of HCC1954 cells to trastuzumab compared to sensitive SK-BR-3 cells in 3-dimensional cell culture. Photos were taken after eight days of trastuzumab treatment.

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BMC Systems Biology

ISSN: 1752-0509

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