Figure 4

Simulation results of our new EGF activated ERK pathway model: (A) ERK Plots: The blue line represents the simulated levels of active ERK over 60 minutes from the model whereas the red line represents the experimentally measured levels of active ERK over 40 minutes from the laboratory; as the lab data is qualitative rather than quantitative, it has been rescaled for comparison to the simulation data. (B) Ras/Rap1 Plots: The red and blue lines represent the simulated levels of active Ras and active Rap1, respectively, over 60 minutes from the model. (C) Knockout Plots: All the lines on this graph represent simulated active ERK levels over 60 minutes. The blue line is the same as in (A). The green line represents active ERK levels when the SOS negative feedback loop is knocked out, whereas the red line represents active ERK levels when receptor degradation is knocked out. (D) Ras/Rap1 Knockout Plots: All the lines on this graph represent simulated active ERK levels over 60 minutes. The blue line is the same as in (A). The green line represents active ERK levels when Ras is knocked out, whereas the red line represents active ERK levels when Rap1 is knocked out.