Deviations from the predictions of the MM equation for diffusion-mediated substrate transport. (Model I) Plot of the Percentage Error in reaction velocity, R
= 100|1 -α
/α|, versus the normalized reaction velocity of the MM equation, α
for 10 enzymes (green) and 100 enzymes (red) with K
= 10 μM in compartments with diameter 100 nm (A) and 50 nm (B). The solid lines show the theoretical predictions, as encapsulated by Eqs. (7) and (8); the data points are obtained by stochastic simulation (see Methods for details).