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Table 2 Maximum Percentage error in reaction velocity from prediction of the MM equation for Model II.

From: Investigating the robustness of the classical enzyme kinetic equations in small intracellular compartments

D/nm K M = 10 μM 100 μM 1000 μM Copy No.
50 225.40 152.83 [291.56] 45.43 10
100 161.59 [331.66] 52.74 [58.39] 6.82 [6.99] 10
200 65.09 8.45 [8.50] 0.88 10
50 32.97 30.17 [61.66] 18.14 100
100 30.78 [66.03] 19.76 [24.52] 5.57 [6.06] 100
200 21.27 6.61 [6.91] 0.85 100
  1. The copy number indicates the total number of enzyme molecules per compartment. Values in bold and in square brackets are those estimated by simulation; the italic values are obtained from the theoretical expressions, Eqs. (7) and (9).