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Table 2 Metabolic flux ratios of T. reesei wild type (wt) and Δcre1 strains in aerobic batch cultures on glucose and on sorbitol with and without sophorose induction of cellulase gene expression.

From: 13C-metabolic flux ratio and novel carbon path analyses confirmed that Trichoderma reesei uses primarily the respirative pathway also on the preferred carbon source glucose

strain

wt

 

Δcre1

 

wt

 

wt

 

Δcre1

 

Δcre1

 

carbon source

glucose

 

glucose

 

sorbitol

 

sorbitol

 

sorbitol

 

sorbitol

 

fraction of total pool (%)

 

sd

 

sd

 

sd

sophorose

sd

control

sd

sophorose

sd

Pep from PPP (UB wo PEPck)

39

2

47

4

36

7

37

7

45

9

46

2

R5P from T3P and S7P

51

1

42

1

72

3

70

1

79

4

79

1

R5P from E4P

25

2

23

1

46

2

48

5

54

3

44

2

Ser from Gly and C1

80

0

85

2

44

2

41

15

54

1

52

0

Gly from CO2 and C1

12

1

14

1

6

2

16

21

8

1

10

3

Oaamit from Pep

35

1

33

2

26

3

26

4

42

5

39

7

MAE (UB)

4

0

9

1

12

2

2

2

6

5

1

nd

MAE (LB)

2

0

6

1

9

1

2

2

4

3

0

nd

  1. sd standard deviation, UB upper bound, LB lower bound