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Table 2 Metabolic flux ratios of T. reesei wild type (wt) and Δcre1 strains in aerobic batch cultures on glucose and on sorbitol with and without sophorose induction of cellulase gene expression.

From: 13C-metabolic flux ratio and novel carbon path analyses confirmed that Trichoderma reesei uses primarily the respirative pathway also on the preferred carbon source glucose

strain wt   Δcre1   wt   wt   Δcre1   Δcre1  
carbon source glucose   glucose   sorbitol   sorbitol   sorbitol   sorbitol  
fraction of total pool (%)   sd   sd   sd sophorose sd control sd sophorose sd
Pep from PPP (UB wo PEPck) 39 2 47 4 36 7 37 7 45 9 46 2
R5P from T3P and S7P 51 1 42 1 72 3 70 1 79 4 79 1
R5P from E4P 25 2 23 1 46 2 48 5 54 3 44 2
Ser from Gly and C1 80 0 85 2 44 2 41 15 54 1 52 0
Gly from CO2 and C1 12 1 14 1 6 2 16 21 8 1 10 3
Oaamit from Pep 35 1 33 2 26 3 26 4 42 5 39 7
MAE (UB) 4 0 9 1 12 2 2 2 6 5 1 nd
MAE (LB) 2 0 6 1 9 1 2 2 4 3 0 nd
  1. sd standard deviation, UB upper bound, LB lower bound
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BMC Systems Biology

ISSN: 1752-0509

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