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Table 3 Metabolic flux ratios of T. reesei wild type (wt) and Δcre1 strains in compared with the corresponding flux ratios observed in the crabtree positive and negative yeasts S. cerevisiae and P. stipitis [26, 27].

From: 13C-metabolic flux ratio and novel carbon path analyses confirmed that Trichoderma reesei uses primarily the respirative pathway also on the preferred carbon source glucose

organism

T. reesei

 

T. reesei

 

T. reesei

 

T. reesei

 

S. cerevisiae

 

S. cerevisiae

 

P.stipitis

 

P.stipitis

 

strain

wt

 

Δ cre1

 

wt

 

Δ cre1

         

carbon source

glucose

 

glucose

 

sorbitol

 

sorbitol

 

glucose

 

glucose

 

glucose

 

glucose

 

culture

batch

 

batch

 

batch

 

batch

 

batch

 

chemostat

 

batch

 

chemostat

 

reference

      

control

 

[26]

 

[27]

 

[27]

 

[27]

 

fraction of total pool (%)

 

sd

 

sd

 

sd

 

sd

 

sd

 

sd

 

sd

 

sd

Pep from PPP (UB wo PEPck)

39

2

47

4

36

7

45

9

0-4

 

40

8

57

9

61

11

R5P from T3P and S7P

51

1

42

1

72

3

79

4

68

2

59

2

57

2

72

2

R5P from E4P

25

2

23

1

46

2

54

3

10

2

33

2

35

2

43

2

Oaamit from Pep

35

1

33

2

26

3

42

5

76

4

31

2

36

2

32

2

MAE (UB)

4

0

9

1

12

2

6

5

25-30

 

<13

 

<6

 

<7

 

MAE (LB)

2

0

6

1

9

1

4

3

nd

nd

nd

nd

nd

nd

nd

nd