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Table 1 Reaction rates used in the R. sphaeroides chemotaxis models

From: A model invalidation-based approach for elucidating biological signalling pathways, applied to the chemotaxis pathway in R. sphaeroides

reaction

parameter(s)

value(s)

(R1) A2 → A2p

k 1

0.03 s-1

(R2) A2p+B1 ⇌ A2 + B1p

0.035(μ Ms)-1, 0.01(μ Ms)-1

(R3) A2p+ Y3 ⇌ A2 + Y3p

0.065 (μ Ms)-1, 0

(R4) A2p+ Y4 ⇌ A2 + Y4p

0.004(μ Ms)-1, 0

(R5) A2p+ Y6 ⇌ A2 + Y6p

0.0006(μ Ms)-1, 0

(R6) A2p+ B2 ⇌ A2 + B2p

0.0035(μ Ms)-1, 0.01(μ Ms)-1

(R7) B1p→ B1

k 7

0

(R8) Y3p→ Y3

K 8

0.08s-1

(R9) Y4p→ Y4

K 9

0.02s-1

(R10) Y6p→ Y6

K 10

0.1s-1

(R11) B2p→ B2

K 11

0.015s-1

(R12) A3A4p+ Y6 ⇌ A3A4+ Y6p

0.1 (μ Ms)-1, 0

(R13) A3A4p+ B2 ⇌ A3A4 + B2p

0.006(μ Ms)-1, 0.07(μ Ms)-1

(R14)A3A4 → A3A4p

k 14

0.02s-1

  1. The reaction rates used for the models determined by fitting phosphotransfer in vitro experiments [16, 31]. For some of these reaction rates the best fit is zero suggesting that these reactions do not occur in vitro.