Spatial expression patterns of PDE1C and G(olf) in the stickleback. Semi-quantitative RT-PCR analysis was performed to assess expression levels and patterns of G-protein subunit alpha olfactory type (G [olf]) and multiple PDE1C genes in stickleback. Plus (+) and minus (-) signs indicate PCR assays using reverse-transcribed cDNA for each tissue type and assays using total RNA without reverse transcription (negative controls), respectively. The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was amplified as a positive control. The overall expression patterns of the genes were essentially similar between the two stickleback individuals investigated.