Evolution of multiple phosphodiesterase isoforms in stickleback involved in cAMP signal transduction pathway

BMC Systems Biology

Table 2 Gene-specific primers for RT-PCR-based expression analysis of PDE1C and G(olf)

Target gene	Sequence (5' → 3')^a	Product length (base pairs)
Stickleback PDE1Ca	Forward: ATGGTGCATTGGTTGACTGA	233
	Reverse: CTCCAGTCGTCCTTGGAGAG
Stickleback PDE1Cb1	Forward: CAAGGGCTTCAAGGTCACAT	152
	Reverse: CCTTTTCCTCCAGGTCTTCC
Stickleback PDE1Cb2	Forward: ACAGACGGACCTCCAACATC	238
	Reverse: TGTTTGCTGTAGCCCACTTG
Stickleback PDE1Cb3	Forward: CAAAGGCTTGTCCCTGCTAC	217
	Reverse: TGGTTCCACCATGAAGTCAA
Stickleback PDE1Cb4	Forward: TGGGCTACAGCAAACACAAG	243
	Reverse: AGTTCTCCAAAGCTCGGTCA
Stickleback PDE1Cb5	Forward: CACTGGCTCAGTGAGTTGGA	185
	Reverse: CTGTGCTGTAGAAGGCGACA
Stickleback PDE1Cb6	Forward: CACTGGCTCAGTGAGTTGGA	239
	Reverse: AGCTCTCTGCTCCACTCGTC
Stickleback PDE1Cb7	Forward: CTCCTTGGAAGTGGGCTACA	230
	Reverse: TGTACAACATGGCGGTGTCT
Stickleback G(olf)	Forward: SAGCAGCAGCTACAACATGG	411
	Reverse: CATTCKCTGGATGATGTCMC

^a Positions with mixed bases are designated by their IUB (the International Union of Biochemistry) codes: R = A/G; Y = C/T; K = G/T; M = A/C; S = G/C; W = A/T.

ISSN: 1752-0509