Figure 2

Gene essentiality Comparison (A) in silico predictions of lethal genes were compared with an experimentally generated screen (in vitro in LB media) that defines an essential metabolic genes set for S. typhimurium. ++,-,† and indicates virulent, avirulent, and attenuated mutants. * The model was not used to distinguish between attenuated and virulent phenotypes as this would require detailed nutrient uptake rate measurements. Instead mutants were only predicted to be virulent (maximum growth rate is greater than zero) or avirulent (maximum growth rate is zero). (B) Comparison of in-silico predicted viability and virulence of metabolic gene mutants that have been implicated in vivo for virulence. (C) In silico prediction statistics for viability and virulence of the in vivo tested mutants (2A) before and after accounting for auxotrophic requirements and nutrient availability in the host.