Figure 2

A number of active regulators varies across conditions. The number of transcription factors active in minimal growth medium as compared to rich medium was the highest, followed by the transition from exponential to stationary phase of growth, during which the cells are known to undergo massive regulatory re-programming, and then sodium azide treatment, which results, among other things, in an interruption of the electron flow chain. Among the amino acid effects, addition of isoleucine appeared to stimulate the highest number of TFs, whereas addition of threonine or glutamate appeared to have no or very little effect on the regulators. The smallest number of differentially active transcription factors was observed in the comparison of chemostat cultures grown at different dilution rates ("WildTypeGrowth").