Figure 2

Overlay of proteomic data sets using the LeishCyc Omics Viewer. Changes in the proteome of L. donovani promastigotes and axenic amastigotes [40] were mapped on to the Cellular Overview using the Pathway Tools Omics Viewer functionality. The supplied gene IDs for L. donovani/L. infantum were converted to the corresponding L. major orthologs prior to mapping. Lines (representing proteins) coded in red represent enzymes that were increased in amastigotes, lines in yellow represent enzymes that were decreased. Selected pathways/pathway groups are numbered as follows: 1 = amino acid biosynthesis; 2 = glycolysis and the tricarboxylic acid (TCA) cycle; 3 = amino acid catabolism; 4 = oxidative phosphorylation; 5 = fatty acid beta oxidation; 6 = pentose phosphate pathway; 7 = gluconeogenesis; 8 = nucleotide biosynthesis; 9 = ergosterol biosynthesis. The arrows in the glycolysis and gluconeogenesis pathways are enzymes specific to these pathways [40].