Figure 2

Deterministic model of the PKA module. (A) Simulation of PKA Model A (blue trace) and PKA Model B (red trace). The cAMP level is 0 initially, and is increased to 270900 molecules per cell (equivalent to 0.015 mM) after 10 seconds, increased to 909000 molecules per cell after 30 seconds, and decreased to 270900 molecules per cell after 60 seconds. (B) Steady state parameter sensitivity analysis carried out on the PKA module. (C) Parameter scan of PKA Model A. The greatest value for PKA difference (79.1%) is achieved when k _cAMPgain = 0.1, k _cAMPloss = 2.2 × 10⁵, k _PKAdiss = 1 × 10⁵, k _RcAMPdiss = 100, k _PKAass = 1000.