Figure 3

Optimisation of the PKA model. The blue trace shows the simulation of PKA Model B, the red trace – PKA Model C, and the green trace – PKA Model D. The cAMP level is 0 initially, and is increased to 270900 molecules per cell (equivalent to 0.015 mM) after 10 seconds, increased to 909000 molecules per cell after 30 seconds, and decreased to 270900 molecules per cell after 60 seconds.