Simulation of two RS latches with varying degrees of crosstalk and cascading signaling pathways. A: Two steps with minimal crosstalk, B: Three steps with minimal crosstalk C: Two steps with moderate crosstalk, and D: Three steps with moderate crosstalk. The same inputs were provided as for the simulations of Figure 4. Stochastic results (solid lines) are superimposed with deterministic results (dashed lines) for substrates PT (light lines) and CT (dark lines). Results have been scaled to range between 0 and 1 as in a digital RS latch. Outputs have been redefined such that PT = P + P' for two step loops or PT = P + P' + P" for three steps, and similarly for other outputs.