Scheme of the transfer workflow. a, Simplified structure of a typical transcriptional gene regulatory interaction database. Using genetic upstream sequences and transcription factor binding site annotations the TFBSs can be re-adjusted and modeled as PWMs for subsequent TFBS predictions. Sequence clustering tools can be applied to the stored genome annotation and gene/protein sequences to gain information about homologous genes/proteins. b, A regulatory interaction can be transferred from a model organism to a closely related species if the regulator as well as the target gene are orthologous and a matching TFBS can be found in the upstream sequence of the orthologous target gene.