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Figure 1 | BMC Systems Biology

Figure 1

From: Model-based extension of high-throughput to high-content data

Figure 1

Workflow for calibrating and linking data from different experiments. The calibration and model are the crucial steps of the procedure described here. Data calibration by a quantile-quantile (QQ) plot allows to translate data from one technique to the units of another measurement. Here, it is necessary to determine quantities with a known relationship from the identical cell population by both methods (blue). Furthermore, a model has to be developed to describe the dependent variables only measurable by one technique in terms of the free variables assessable by both techniques (green). Then, additional cell populations can be measured by the high-throughput technique only and the information content of the data can be increased via the calibration and the model (dashed arrows, red) to be finally combined with cell population data for mathematical modeling by an ordinary differential equation (ODE) model (transparent red). General terms are shown in bold letters, the specific case of the example system is given below. Steps shown in transparent colors are not subject of this study.

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