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Table 2 Experimental data and model consistency

From: Validation of a constraint-based model of Pichia pastoris metabolism under data scarcity

Ref*

μ

QGlu

QGly

QMet

Qet

OUR

CPR

QP

Yields Exp./Theo.

Consistency**

 

Cmol·kg-1·h-1

mol·kg-1·h-1

"

"

"

"

"

mg·g-1·h-1

Cmol DW/mol

"

ϕ

π

To π = 1

D1

3.86

0.97

0.00

0.00

0.00

2.02

2.07

0.020

3.98

< 6.62

0.03

1.00

2%

A1

1.88

0.00

1.09

0.00

0.00

2.16

1.56

0.000

1.73

< 2.46

0.28

1.00

7%

A2

2.07

0.00

0.95

0.63

0.00

2.70

1.70

0.001

1.31

< 2.25

1.20

0.73

12%

A3

1.72

0.00

0.74

1.48

0.00

3.90

2.10

0.014

0.77

< 2.25

2.81

0.25

20%

A4

2.02

0.00

0.57

2.33

0.00

4.85

2.21

0.024

0.70

< 2.25

5.36

0.09

29%

B1

6.17

0.00

2.75

0.00

0.00

3.62

2.35

0.000

2.24

< 2.46

0.07

1.00

4%

B2

6.18

0.00

2.77

1.87

0.00

7.19

4.18

0.001

1.51

< 2.25

0.88

0.82

12%

B3

6.24

0.00

2.23

2.73

0.00

7.20

3.60

0.012

1.26

< 2.25

2.34

0.32

19%

C1

2.32

0.00

0.67

2.01

0.00

3.21

1.77

0.012

0.78

< 2.25

0.15

1.00

5%

C2

2.32

0.00

0.28

3.18

0.00

3.79

2.09

0.021

0.63

< 2.25

0.74

1.00

10%

C3

2.32

0.00

0.00

4.02

0.00

4.22

2.33

0.022

0.52

< 0.82

1.55 > 10

0.49

15%

Random

0-10

0-10

0-10

0-10

0-10

0-10

0-10

-

  

99% > 10

< 0.1 99%

-

Random

1.5-6

0-2

0-2.7

0-2.7

0-0.1

2.1-7.2

1.5-4

-

  

86%

< 0.1 95%

-

  1. *All the datasets correspond to continuous fermentations in defined chemical media. Further detail can be found in D: Dragosits et al. [22]; A: Solà et al. [21]; B: Solà et al. [21]; C: Jungo et al. [19]. Citrate and Pyruvate are assumed not to be produced nor consumed except for dataset D1 in which citrate is consumed at 0.007 Cmol·kg-1·h-1.
  2. **Minimized sum of squared residuals (ϕ), possibility of the most possible flux vector (P) and degree of measurements uncertainty to P = 1.