Experimental and in silico growth characteristics of Francisella on carbohydrates. A. Growth curves of Francisella follow monod kinetics. Cells were grown in Chamberlain medium supplemented with the respective carbon source as indicated (FRU; fructose, ARA: arabinose, XYL: xylose, RIB: ribose, GLY: glycerol, GLU: glucose). Optical density (600 nm) was measured every 120 minutes. B. Final biomass yields on different carbon sources. Cells were grown in Chamberlain medium supplemented with carbon sources as in A. Yield is expressed in dry cell weight (DCW). C. Comparison of growth rates in Chamberlain media supplemented with carbon sources as indicated were determined experimentally and calculated in silico under aerobic conditions. Specific growth rates on glucose were used to determine the Um and Ks values. D. Growth rates in Chamberlain medium supplemented with carbon sources as indicated were determined experimentally and calculated in silico under microaerobic conditions. Robustness analysis using i RS605 depicts aerobicity based on ranges of oxygen uptake rates and the corresponding effect on growth.