Figure 5

Selected metabolic transcriptome of Francisella in varying environments. A. Bacteria were grown in Chamberlain medium supplemented with carbon sources as indicated (Fru: Fructose, Ara: Arabinose, Xyl: Xylose, Rib: Ribose, Gly: Glycerol, Glu: Glucose, C: Chamberlain media only). Expression of 75 selected metabolic genes was measured using RT-PCR and GeXP (described in detail in Materials and Methods). Data were normalized to the house-keeping gene FTL_001 and are presented relative to expression in glucose media. B. Macrophages were infected with Francisella for 1 h and for 24 h. Expression of selected metabolic genes was quantitatively measured as described in A. Data were normalized to the house-keeping gene FTL_001 and are presented relative to gene expression in macrophage 1 h after infection. C. Composite Heat Map of the selected Francisella metabolic transcriptome. Data from A and B were clustered based on environments and functional categories of the genes.