Skip to main content

Table 2 Fold changes in metabolic enzyme expression

From: Transcriptional regulation of respiration in yeast metabolizing differently repressive carbon substrates

enzyme

man

gal

pyr

enzyme

man

gal

pyr

TCA cycle

respiratory chain without ATPase

Yea6p

nsc

nsc

nsc

Ndi1p

nsc

2.4

2.1*

Pda1p

nsc

nsc

2.8

Nde1p

nsc

nsc

nsc

Pdb1p

nsc

3.0

2.7

Cyt1p

nsc

nsc

nsc

Lat1p

nsc

nsc

nsc

Cor1p

nsc

3.8

6.0

Lpd1p

nsc

3.1

5.1*

Qcr2p

nsc

nsc

5.9

Pdx1p

nsc

nsc

0‡

Qcr6p

nsc

3.7

2.8*

Cit1p

nsc

6.0

14.1

Qcr7p

nsc

2.5

nsc

Cit3p

nsc

nsc

nsc

Qcr8p

0.2*

nsc

0.2

Aco2p

nsc

nsc

0.3

Qcr9p

nsc

nsc

nsc

Idh1p

nsc

2.1

3.0

Qcr10p

nsc

nsc

nsc

Idh2p

nsc

2.5

3.6

Rip1p

nsc

ng

ng

Idp1p

nsc

nsc

0.6

Cox4p

nsc

3.6*

5.1

Kgd1p

nsc

2.7

2.9

Cox5ap

nsc

nsc

nsc

Kgd2p

nsc

nsc

nsc

Cox5bp

nsc

nsc

nsc

Lsc1p

nsc

nsc

2.5

Cox6p

nsc

3.9

5.8*

Lsc2p

nsc

nsc

nsc

Cox7p

nsc

5.0

nsc

Sdh2p

0‡

4.8

4.6**

Cox8p

nsc

nsc

nsc

Sdh4p

nsc

nsc

2.8*

Cox9p

nsc

nsc

nsc

Fum1p

nsc

3.1

6.8

Cox12p

nsc

ng

ng

Mdh1p

nsc

3.9

7.4*

ethanol production

Mae1p

nsc

0.4**

0‡

Pdc1p

0.9

0.6

0.4

Pdc5p

nsc

nsc

nsc

    

nsc = not significantly changed

Pdc6p

nsc

nsc

nsc

ng = no growth

Adh2p

nsc

nsc

16.9

0‡ = under detection limit

Adh4p

0*‡

0.1*

0.2*

* = p-value 0.06-0.10

Adh5p

nsc

0.1

0.1

** = p-value 0.11-0.12

Sfa1p

nsc

nsc

nsc

  1. Fold changes in enzymes expressed on mannose (man), galactose (gal) or pyruvate (pyr) compared to growth on glucose (glc) (alternative carbon source/glucose). Expression levels are determined during exponential growth in batch with strains containing a single enzyme-GFP construct. Significant changes were assigned when the p-value was ≤ 0.12 (calculated from three independent experiments) compared to the enzyme expression during growth on glucose.